Identification of environmental Vibrio cholerae non 01 non 139 by MALDITOF MS
DOI:
https://doi.org/10.24039/rtb20232111529Keywords:
bacteria, cholera, proteins, review, serotypeAbstract
Most Vibrio species identification methods take many days to deliver results, therefore it is important that research can be done on rapid and accurate detection and identification techniques. MALDI-TOF MS has proven to be a simple tool to identify and differentiate pathogens. The objective of this research was to recognize the available bibliographic information on the identification of Vibrio cholerae species non 01 non 139 of environmental origin by MALDI TOF MS. The Vibrio genera includes more than thirty species found in aquatic environments, some species cause diseases in both marine and human species. One of these organisms is V. cholerae, one of the most important species due to the production of cholera toxin (CT) which interrupts the transport of ions in the cells of the intestinal epithelium. The number of case reports involving V. cholerae no 01 no 139 in extraintestinal infections and potentially fatal bacteremia in healthy patients has increased and, despite the existence of molecular identification techniques such as PCR, sequencing, and gel electrophoresis pulsed-field, the MALDI TOF MS technique has been gaining ground in bacteriology laboratories in hospitals due to its speed and ease of use. There are studies that cover the detection of V. cholerae in MALDI-TOF MS, focusing on food and environment, however, when performing the search key, no specific studies on the detection of V. cholerae non 01 non 139 of environmental origin by MALDI TOF MS were found with the exception of a case report, presence in blood isolates, reviews of reports that indicate the importance of these microorganisms in the cause bacteremia and the importance of this technique for the future of bacterial identification. Therefore, is recognized that there is a need to continue producing new information to contribute to the implementation of genomic surveillance capable of predicting future epidemic outbreaks.
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Remuneración: La cesión de los derechos patrimoniales de autor que mediante este contrato se hace será a título gratuito.
Condiciones y legitimidad de los derechos: El AUTOR-CEDENTE garantiza que es propietario integral de los derechos de explotación de la(s) obra(s) y en consecuencia garantiza que puede contratar y transferir los derechos aquí cedidos sin ningún tipo de limitación por no tener ningún tipo de gravamen, limitación o disposición. En todo caso, responderá por cualquier reclamo que en materia de derecho de autor se pueda presentar, exonerando de cualquier responsabilidad al CESIONARIO.
Licencia de acceso abierto: El AUTOR-CEDENTE autoriza que manuscrito publicado en la Revista Científica The Biologist (Lima) (versión Impresa ISSN 1816-0719, versión en línea ISSN 1994-9073) permanece disponible para su consulta pública en el sitio web http://revistas.unfv.edu.pe/index.php/rtb/index y en los diferentes sistemas de indexación y bases de datos en las que la revista tiene visibilidad, bajo la licencia Creative Commons, en la modalidad Reconocimiento-No comercial- Sin Trabajos derivados –aprobada en Perú, y por lo tanto son de acceso abierto. De ahí que los autores dan, sin derecho a retribución económica, a la Escuela Profesional de Biología, Facultad de Ciencias Naturales y Matemática de la Universidad Nacional Federico Villarreal (EPB - FCCNM - UNFV), los derechos de autor para la edición y reproducción a través de diferentes medios de difusión.
