Identificación de Vibrio cholerae no 01 no 139 ambiental por MALDITOF MS

Autores/as

  • Alejandra E. Cuneo Universidad Nacional Federico Villarreal. Facultad de ciencias Naturales y Matemática. Av Rio Chepen S/N, El Agustino, Lima, Perú. Instituto Nacional de Salud. Laboratorio de Referencia en Enteropatógenos. Jirón Cápac Yupanqui 1400, Jesús María, Lima, Perú. https://orcid.org/0000-0003-2449-1660
  • Armando Vélez-Azañero Universidad Nacional Federico Villarreal. Facultad de ciencias Naturales y Matemática. Av Rio Chepen S/N, El Agustino, Lima, Perú.Municipalidad Distrital de Lurín. Gerencia de Servicios públicos y gestión ambiental. Jr. Grau 399. Lurín, Lima, Perú. https://orcid.org/0000-0003-4246-1502

DOI:

https://doi.org/10.24039/rtb20232111529

Palabras clave:

bacteria, cólera, proteínas, revisión, serotipo

Resumen

La mayoría de los métodos de identificación de especies de Vibrio tardan muchos días en ofrecer resultados, frente a ello, la técnica MALDI-TOF MS ha demostrado ser una herramienta rápida y sencilla para identificar y diferenciar patógenos. El objetivo de la investigación fue reconocer la información bibliográfica disponible sobre la identificación de especies de Vibrio cholerae no 01 no 139 de procedencia ambiental por MALDI TOF MS. El género Vibrio incluye más de treinta especies comúnmente encontradas en ambientes acuáticos donde algunas causan enfermedades en especies marinas y el hombre. V. cholerae, una de las especies más importantes debido a la producción de la toxina colérica que interrumpe el transporte de iones en las células del epitelio intestinal. Se han incrementado el número de reporte de casos que incluyen a V. cholerae no 01 no 139, en infecciones extra intestinales y bacteriemias potencialmente fatales en pacientes saludables y, a pesar de que existen técnicas de identificación molecular como el PCR, secuenciamiento y electroforesis en gel por campo pulsado, la técnica de MALDI TOF MS ha ido ganando terreno en laboratorios de bacteriología en hospitales debido a su rapidez y facilidad de uso.  Existen estudios que abarcan el tema de detección de V. cholerae en MALDI-TOF MS, enfocándose en alimentos y medioambiente, sin embargo, al realizar la búsqueda, no se encontraron estudios específicos sobre la detección de V. cholerae no 01 no 139 de procedencia ambiental por MALDI TOF MS a excepción de un reporte de caso, presencia en aislamientos sanguíneos, revisiones de reportes que indican la importancia de estos microorganismos en la causa de bacteriemias y la importancia de esta técnica para el futuro de la identificación bacteriana. Por tanto, se reconoce la necesidad de seguir produciendo nueva información para contribuir en la implementación de una vigilancia genómica capaz de predecir futuros brotes epidémicos.

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2023-01-20

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Cuneo, A. E. ., & Vélez-Azañero, A. . (2023). Identificación de Vibrio cholerae no 01 no 139 ambiental por MALDITOF MS. The Biologist, 21(1), 87–98. https://doi.org/10.24039/rtb20232111529

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