MORPHOLOGICALAND MORPHOMETRIC STUDIES ON PROTOSCOLECES ROSTELLAR HOOKS OF ECHINOCOCCUS GRANULOSUS FROM PERU VISUALIZED BY SEVERAL MICROSCOPIC TECHNIQUES

Authors

  • Fernanda Almeida B Laboratório de Helmintos Parasitos de Vertebrados, Departamento de Helmintologia, Instituto Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, 21040-900, Rio de Janeiro, Brasil. https://orcid.org/0000-0003-4784-6540
  • Rosângela Rodrigues-Silva Laboratório de Helmintos Parasitos de Vertebrados, Departamento de Helmintologia, Instituto Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, 21040-900, Rio de Janeiro, Brasil. https://orcid.org/0000-0001-7458-6030
  • Renata Neves H Laboratório de Helmintos Parasitos de Vertebrados, Departamento de Helmintologia, Instituto Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, 21040-900, Rio de Janeiro, Brasil. https://orcid.org/0000-0002-5442-0030
  • Margareth Gonçalves Ml Laboratório de Helmintos Parasitos de Vertebrados, Departamento de Helmintologia, Instituto Oswaldo Cruz, Avenida Brasil 4365, Manguinhos, Rio de Janeiro, 21040-900, Rio de Janeiro, Brasil. https://orcid.org/0000-0002-8873-6519
  • Elizabeth Romani L S Departamento de Parasitologia, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Peru. https://orcid.org/0000-0001-8179-1657
  • José Roberto Machado-Silva Laboratório Romero Lascasas Porto, Departamento de Microbiologia, Imunologia e Parasitologia, Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Rua Prof. Manoel de Abreu 444/5o andar, Vila Isabel, Rio de Janeiro, 20511-070, Brasil. https://orcid.org/0000-0003-3085-0228

DOI:

https://doi.org/10.24039/rnh2009321114

Keywords:

Echinococcus granulosus, microscopycal techniques, morphology, morphometry, Peru, rostellar hooks

Abstract

This study was undertaken to expand the current knowledge of the morphology and morphometry of rostellar hooks of protoscoleces from the metacestode E. granulosus. Protoscoleces were isolated from livers and lungs of naturally infected ovines and bovines obtained from abattoirs in Peruvian provinces (Arequipa, Cuzco, Puno, Huancavelica and Junin). Brightfield microscopy, confocal laser scanning microscopy, differential interference contrast and variable pressure scanning electron microscopy were used. Morphometry was made using computer image analysis. The application of these assay indicated that the large hooks frequently had thin guards and an irregular surface between the guard and handle. Data also showed that the small hooks presented rounded and stout guards. The blade did not show any relevant feature. No clear morphological distinction was observed between large and small hooks. Phenotypical polymorphism was evident in the shape and size of hooks. In conclusion, the current data show that large and small rostellar hooks have morphological polymorphism. Because the application of this knowledge for taxonomic study is limited, for this end morphometry techniques are required. Our study demonstrated the usefulness of combining conventional and new morphological tools to help to solve unresolved matters with regards to rostellar hooks features.

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Published

2009-06-09

How to Cite

Almeida B, F., Rodrigues-Silva, R. ., Neves H, R. ., Gonçalves Ml, M. ., Romani L S, E. ., & Machado-Silva, J. R. . (2009). MORPHOLOGICALAND MORPHOMETRIC STUDIES ON PROTOSCOLECES ROSTELLAR HOOKS OF ECHINOCOCCUS GRANULOSUS FROM PERU VISUALIZED BY SEVERAL MICROSCOPIC TECHNIQUES. Neotropical Helminthology, 3(2), 65–72. https://doi.org/10.24039/rnh2009321114

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Artículos Originales