image/svg+xml217Ectoparasites (Phthiraptera) In the Chilean FlamingoNeotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023Neotropical HelminthologyNeotropical Helminthology, 2023, vol. 17 (2), 217-224ORIGINAL ARTICLE / ARTÍCULO ORIGINALGENARCHELLA PARVATRAVASSOS, ARTIGAS & PEREIRA, 1928 (DIGENEA: DEROGENIDAE) IN FISH FROM TWO ENVIRONMENTS IN SOUTHERN BRAZIL: MORPHOLOGY AND COMMENTS ON HOST’S CHECKLISTGENARCHELLA PARVATRAVASSOS, ARTIGAS & PEREIRA, 1928 (DIGENEA: DEROGENIDAE) EM PEIXES EM DOIS AMBIENTES NO SUL DO BRASIL: MORFOLOGIA E COMENTÁRIOS SOBRE A LISTA DE HOSPEDEIROSGENARCHELLA PARVATRAVASSOS, ARTIGAS & PEREIRA, 1928 (DIGENEA: DEROGENIDAE) EN PECES EN DOS AMBIENTES DEL SUR DE BRASIL: MORFOLOGÍA Y COMENTARIOS SOBRE LA LISTA DE HOSPEDADORESMoisés Gallas1,*& Laura Roberta Pinto Utz1ISSN Versión Impresa 2218-6425 ISSN Versión Electrónica 1995-1403DOI: https://dx.doi.org/10.24039/rnh20231721658Volume 17, Number 2 (jul - dec) 2023Este artículo es publicado por la revista Neotropical Helminthology de la Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú auspiciado por la Asociación Peruana de Helmintología e Invertebrados Af nes (APHIA). Este es un artículo de acceso abierto, distribuido bajo los términos de la licencia Creative Commons Atribución 4.0 Internacional (CC BY 4.0) [https:// creativecommons.org/licenses/by/4.0/deed.es] que permite el uso, distribución y reproducción en cualquier medio, siempre que la obra original sea debidamente citada de su fuente original.1Laboratório de Ecologia Aquática, Escola de Ciências da Saúde e da Vida, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, 90619-900, Rio Grande do Sul, Brazil.* Corresponding author: mgallas88@gmail.comMoisés Gallas: https://orcid.org/0000-0003-4525-009XLaura Roberta Pinto Utz: https://orcid.org/0000-0002-4343-4672ABSTRACTGenarchellaparvaTravassos, Artigas & Pereira, 1928 has been reported mainly in Characidae hosts. T e morphology of this species has been known based on specimens collected mainly by Travassos and researchers (20s and 60s decades) and also from specimens in Argentina. T e goal of this study was to describe the morphology of G. parva, provide the parameters of the infections from dif erent f sh species, and discuss the host’s checklist from Brazil. Specimens of G. parvawere collected from species of Astyanax Baird & Girard, 1854 and Psalidodon Eigenmann, 1911 in Jacuí Delta (north of Lake Guaíba, in the municipality of Porto Alegre) and Lagoon Itapeva (municipality of Terra de Areia), extreme south of Brazil. T e localization of the genital pore of G. parvaand Genarchella fragilisLunaschi, 1990 was compared and discussed. T e prevalence of G. parvawas higher in Psalidodonaf . fasciatus(Cuvier, 1819) (12.9%) from Itapeva Lagoon and AstyanaxhenseliMelo & Buckup, 2006 (11.43%) from Jacuí Delta than other species examined. A host’s checklist of G. parvain Brazil is provided and two previous reports are discussed. T is study is the f rst report of G. parvain A. henseliand A.lacustrisfrom southern Brazil, while Itapeva Lagoon is considered a new locality record for G. parva.Keywords:Biodiversity – characids – digenetic trematode – Halipeginae – helminths – Icthyoparasitology
image/svg+xml218Neotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023Gallas et al.RESUMOGenarchellaparvaTravassos, Artigas & Pereira, 1928 foi registrada principalmente em hospedeiros da família Characidae. A morfologia desta espécie é conhecida a partir de espécimes coletados principalmente por Travassos e outros pesquisadores (nas décadas de 20 e 60) e também por espécimes da Argentina. O objetivo do presente estudo foi descrever a morfologia de G. parva, fornecer os parâmetros das infecções em diferentes espécies de peixes e discutir a lista de hospedeiros do Brasil. Espécimes de G. parvaforam coletados em espécies de Astyanax Baird & Girard, 1854 e Psalidodon Eigenmann, 1911 do Delta do Jacuí (limite norte do Lago Guaíba, no município de Porto Alegre) e Lagoa Itapeva (município de Terra de Areia), no extremo sul do Brasil. A localização do poro genital de G. parvae Genarchella fragilisLunaschi, 1990 foi comparada e discutida. A prevalência de G. parvafoi elevada em Psalidodonaf. fasciatus(Cuvier, 1819) (12,9%) da Lagoa Itapeva e AstyanaxhenseliMelo & Buckup, 2006 (11,43%) do Delta do Jacuí em relação as outras espécies examinadas. Uma lista de hospedeiros de G. parvano Brasil é fornecida e dois registros foram discutidos. Este estudo é o primeiro registro de G. parvaem A. henselie A.lacustrispara o sul do Brasil, enquanto que a Lagoa Itapeva é considerada como novo registro de localidade para G. parva.Palavras-chave:Biodiversidade – caracídeos – Halipeginae – helmintos – Ictioparasitologia – trematódeo digenéticoRESUMENGenarchella parvaTravassos, Artigas & Pereira, 1928 ha sido mayormente registrada en hospedadores pertenecientes a la familia Characidae. La morfología de esta especie se conoce a partir de especímenes recolectados principalmente por Travassos y otros investigadores (en las décadas de 1920 y 1960), así como de especímenes procedentes de Argentina. El propósito de este estudio es describir la morfología de G. parva, proporcionar los parámetros de infecciones en diferentes especies de peces y discutir la lista de hospedadores en Brasil. Se recolectaron especímenes de G. parvade las especies de AstyanaxBaird & Girard, 1854, y PsalidodonEigenmann, 1911, en el Delta do Jacuí (límite norte del Lago Guaíba, en el municipio de Porto Alegre) y en la Lagoa Itapeva (municipio de Terra de Areia), ubicados en el sur de Brasil. Además, se comparó y discutió la ubicación del poro genital de G. parvaen relación con Genarchella fragilisLunaschi, 1990. La prevalencia de G. parvafue notablemente alta en Psalidodon af. fasciatus(Cuvier, 1819) (12,9%) de Lagoa Itapeva y Astyanax henseliMelo & Buckup, 2006 (11,43%) del Delta del Jacuí en comparación con otras especies examinadas. Se presenta una lista detallada de hospedadores de G. parvaen Brasil, y se analizan dos registros en particular. Este estudio constituye el primer registro de G. parvaen A. henseliy A. lacustrispara el sur de Brasil, mientras que la Lagoa Itapeva se considera un nuevo registro de localidad para G. parva.Palabras clave: Biodiversidad – carácidos – Halipeginae – helmintos – Ictioparasitología – tremátodos digéneos
image/svg+xml219Genarchella parvain fsh from southern BrazilNeotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023INTRODUCTIONGenarchellaTravassos, Artigas & Pereira, 1928 was proposed to accommodate GenarchellagenarchellaTravassos, Artigas & Pereira, 1928 (type species) and GenarchellaparvaTravassos, Artigas & Pereira, 1928 (Travassos et al., 1928). In South America, seven species of Genarchellahave been reported: Genarchella astyanacis(Watson, 1976) Scholz, Vargas-Vazquez & Salgado-Maldonado, 1995, Genarchella fragilisLunaschi, 1990, G.genarchella, Genarchella isabellae(Lamothe-Argumedo, 1977) Kohn, Fernandes, Gibson & Fróes, 1990, G.parva, Genarchella porzanae(Nasir & Gómez, 1977) Gibson, 2002, and Genarchella tropica(Manter, 1936) Szidat, 1954 (Kohn et al., 2007, 2011; Virgilio et al., 2022), although only G. fragilisand G. porzanaehave not been reported in hosts from Brazil.Until now, the morphology of G. parvahas been known based on specimens mainly collected in the past by Travassos and other researchers (Travassos et al., 1928; Kloss, 1966; Kohn & Fernandes, 1988; Kohn et al., 1990) and from specimens collected in Argentina (Lunaschi, 1990). In Brazil, G. parvahas been reported in diferent hosts (Kohn et al., 2007) such as Astyanaxbimaculatus(Linnaeus, 1758) and Psalidodonaf. fasciatus(Cuvier, 1819) from specimens collected in the state of São Paulo and Espírito Santo (Travassos et al., 1928; Kloss, 1966; Kohn et al., 1990), and P. af. fasciatusfrom Lake Guaíba, state of Rio Grande do Sul, southern Brazil (Fortes & Hofmann, 1999). Some of characid fshes previously classifed in AstyanaxBaird & Girard, 1854, were currently transferred to Psalidodon Eigenmann, 1911 (Psalidodoneigenmanniorum(Cope, 1894) and P. af. fasciatus) (Terán et al., 2020).Te present study is a part of the studies carried out on the helminth fauna of the species of Astyanax(AstyanaxhenseliMelo & Buckup, 2006 and Astyanax lacustris(Lütken, 1875)) and Psalidodonspecies (P. eigenmanniorumand P. af. fasciatus) from southern Brazil (Gallas & Utz, 2019abc). In this paper, we describe the morphology of G.parva, present the parameters of infections in three fsh species, and provide new hosts reports, including a discussion concerning the host’s checklist of previous reports in Brazil.MATERIAL AND METHODSSpecimens of fsh were acquired from fshermen in two localities between 2017 and 2020: A. henseli(n = 35), A. lacustris(n = 42), P. eigenmanniorum(n = 29), and P.af. fasciatus(n = 54) were collected in Jacuí Delta, in the locality of Pintada Island (30º17’11’’S, 51º18’01”W), which represents the limit north of the Lake Guaíba in the municipality of Porto Alegre. Additionally, specimens of A.lacustrisand P. af. fasciatus(n = 31 each) were collected in Lagoon Itapeva (29º36’16”S, 49º59’28”W), municipality of Terra de Areia, in the state of Rio Grande do Sul, extreme south of Brazil. Fish were collected with seine nets or with the aid of hooks, packed in plastic bags, and transported in ice coolers to the laboratory for posterior necropsy. Host specimens were identifed following Bertaco & Lucena (2010), Lucena et al. (2013) and Lucena & Soares (2016).Procedures of helminth collection and staining were made according to Gallas & Utz (2019abc). Measurements represent the range followed by mean ± standard deviation, shown in micrometers (µm) unless otherwise stated. Figures were made using a Olympus BX50 microscope, scanned and prepared using CorelDraw X4® and Adobe’s Photoshop® CS2. Prevalence, mean intensity and abundance of infections follow Bush et al. (1997). Helminth specimens were deposited in the Helminthological Collection of the Natural Sciences Museum (Coleção Helmintológica do Museu de Ciências Naturais, CHMU) at the Lutheran University of Brazil in Canoas, Rio Grande do Sul, Brazil, and in the Protist Collection of the Museum of Science and Technology (Coleção de Protistas do Museu de Ciências e Tecnologia, MCTP) at the Pontifcal Catholic University of Rio Grande do Sul in Porto Alegre, Rio Grande do Sul, Brazil.Ethic aspects: Te study was approved by the Institutional Committee (Project # 7398). All fsh were purchased dead.RESULTSGenarchella parvaTravassos, Artigas & Pereira, 1928 (Figs 1 and 2).
image/svg+xml220Neotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023Gallas et al.Figure 2. Photomicrographs of Genarchella parvaTravassosFigure 1.Diagram of specimen in totoof Genarchella parvaTravassos, Artigas & Pereira, 1928, ventral view, scale bar = 200 µm.Figure 2. Photomicrographs of Genarchella parvaTravassos, Artigas & Pereira, 1928. A.Specimen in toto; ventrolateral view, scale bar = 250 µm. B.Region of ventral sucker, lateral view, scale bar = 200 µm. C.Eggs, showing the single flament (asterisks), ventral view, scale bar = 25 µm.
image/svg+xml221Genarchella parvain fsh from southern BrazilNeotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023Description based on nine specimens. Derogenidae, Halipeginae. Tegument without spines. Body elongated, 1.42‒3.6 mm (2.5 ± 0.7 mm; n = 9) long, 0.25‒0.47 mm (0.38 ± 0.08 mm; n = 9) wide. Oral sucker 150‒220 (184 ± 26; n = 9) long, 150‒260 (193 ± 34; n = 9) wide. Ventral sucker campaniform, 260‒480 (367 ± 72; n = 9) long, 230‒400 (332 ± 54; n = 9) wide, and 0.53‒1.22 mm (0.83 ± 0.22 mm) from anterior end. Ratio of total body length/oral sucker 1:13.9; ratio of total body length/ventral sucker 1:6.7; ratio of oral sucker/ventral sucker 1:0.5. Pharynx 60‒100 (83 ± 13; n = 9) long, 60‒110 (80 ± 16; n = 9) wide. Esophagus indistinct, bifurcation of the intestinal caeca 230‒330 (283 ± 44; n = 7) from anterior end; intestinal caeca ending next to the vitellarium.Testes oval, oblique, intercaecal, of similar size, 80‒250 (178 ± 50; n = 9) long, 70‒220 (150 ± 49; n = 9) wide. External seminal vesicle sacculiform, 70‒110 (86 ± 15; n = 5) long, 30‒45 (37 ± 7; n = 5) wide. Sinus-sac 30‒60 (47 ± 11; n = 6) long, 30‒55 (44 ± 9; n = 6) wide. Sinus-organ not observed. Genital pore ventral, in the feld of the pharynx. Ovary oval, elongated, intercaecal, post-testicular, anterior to vitellarium, 90‒350 (209 ±98; n = 7) long, 90‒160 (136 ± 22; n = 7) wide. Mehlis’ gland and Laurer’s canal not observed. Uterus mainly in the hindbody, occupying the middle and superior area of the ovary, inter and extracaecal. Vitellarium formed by two relatively symmetrical and oval masses, 100‒230 (164 ± 40; n = 9) long, 80‒130 (104 ± 21; n = 9) wide. Eggs with long, single flament at anopercular pole, 22‒47 (38 ± 8; n = 9) long, 13‒25 (21 ± 4; n = 9) wide. Excretory vesicle not observed, excretory pore terminal.Taxonomic summary:Synonyms (according Kohn et al., 2007): Genarchella tropicaManter, 1936 by Szidat (1954), Genarchella genarchellaTravassos, Artigas & Pereira, 1928 by Travassos et al. (1964), Halipegus tropicus(Manter, 1936) by Kloss (1966), Halipegus cryptorchisby Mañé-Garzon & Gascón (1973), Halipegus parvaand Halipegussp. by Kohn & Fernandes (1987, 1988), and Genarchella szidatiby Hamann (1989).Hosts: AstyanaxhenseliMelo & Buckup, 2006 (new host record), Astyanax lacustris(Lütken, 1875) (new host record), andPsalidodonaf. fasciatus(Cuvier, 1819).Localities: Pintada Island, municipality of Porto Alegre, and Lagoon Itapeva, municipality of Terra de Areia (new locality record).Site of infection: stomach.Helminth specimens deposited: CHMU 319, MCTP 09.DISCUSSIONSpecies of Genarchellapresent diferences concerning the general morphology of the body, shape of the ventral sucker and the degree of development of the sinus-organ (Kohn & Fernandes, 1988; Kohn et al., 1990). Te species reported in South America with ventral sucker campaniform are G. parvaand G. fragilis(Kohn et al., 1990; Lunaschi, 1990; Scholz et al., 1995), and they are considered morphologically similar. Lunaschi (1990) described G. fragilisand distinguished it from G. parvaby body delicate and fragile and by the position of the genital pore. However, body delicate and fragile is not very informative to determine a species, and the procedures of host collection and helminth preparation could infuence the conservation and quality of the specimens, mainly in the body shape.Te position of genital pore in G. fragiliswas described as in the below limit of the pharynx or more posterior (Lunaschi, 1990). When comparing the descriptions and illustrations of G. parvain diferent studies, the location of the genital pore and consequently the sinus-sac appear to vary: they could be found in the feld of the pharynx (Kohn & Fernandes, 1988; Kohn et al., 1990; Lunaschi, 1990), as well as above (Kohn & Fernandes, 1988; Kohn et al., 1990; Scholz et al., 1995) or below (Travassos et al., 1928; Kohn et al., 1990; Lunaschi, 1990) the limit of the pharynx. Te specimens found in the present study presented those traits mostly in the feld of the pharynx or below the limit of the pharynx. Terefore, due the variation in G. parva, the specimens of G. fragilisshould be reexamined to confrm the position of this trait in order to recognize or not both species.In general, the measurements of G. parvafound in the present study are similar to those found in the literature (Kohn & Fernandes, 1988; Kohn et al., 1990; Lunaschi, 1990). Te parameters of the infections showed a higher prevalence (12.9%) in P. af. fasciatusfrom Itapeva Lagoon than other fsh species examined (Table 1). Although with some diferences in the life cycle of the species of Genarchella(Yamaguti, 1975; Ditrich et al., 1997), possibly the infections in the examined fsh in the present study occurred through random ingestion of the cercariae due the opportunistic food habit of the hosts (Vilella et al., 2002), and may explain the diferences in the parameters of the infections.
image/svg+xml222Neotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023Gallas et al.In Brazil, G. parvahave been reported from diferent hosts, but mainly in Characidae (Table 2). However, two host species deserve attention: Acestrorhynchusfalcatus(Bloch, 1794) and Oligosarcushepsetus(Cuvier, 1829). Travassos et al. (1928) found G. parvain Acestrorhamphussp. (type host) in the state of São Paulo (type locality), a host posteriorly reported as A. falcatusby Kohn & Fernandes (1988), Kohn et al. (1990) and Kohn et al. (2007). However, A. falcatusoccurs in the Amazon and Table 1.Parameters of the infections of Genarchella parvaTravassos, Artigas & Pereira, 1928 in diferent fsh.Host species and localitiesPrevalence (%)Mean intensity (helminth/host)Mean abundance (helminth/host)A. henseli(from Jacuí Delta)11.432.250.26A. lacustris(from Jacuí Delta)2.3820.04A. lacustris(from Itapeva Lagoon)6.4510.06P. af. fasciatus(from Itapeva Lagoon)12.91.50.19Orinoco River basins and rivers of Guyana (Buckup et al., 2007). Fortes & Hofmann (1985) reported G. parvain O. hepsetusfrom Lake Guaíba, but the host species only occurs in southeastern Brazil rivers (Buckup et al., 2007). In both cases, these records must be attributed to another host species. For future studies, and to avoid loss of information, researchers must follow a key to the host species or deposit voucher specimens of hosts.Table 2. Previous records of Genarchella parvaTravassos, Artigas & Pereira, 1928 from hosts in Brazil.Host familiesHost speciesLocalitiesReferencesAcestrorhynchidaeAcestrorhynchusfalcatus(Bloch, 1794)*Rio Moji-Guaçu, Pirassununga, state of São PauloTravassos et al. (1928); Kohn & Fernandes (1988); Kohn et al. (1990)CharacidaeAstyanaxbimaculatus(Linnaeus, 1758)state of Espírito SantoKohn et al. (1990)Psalidodonaf. fasciatus(Cuvier, 1819)Rio Moji-Guaçu, Pirassununga, state of São PauloKloss (1966); Kohn et al. (1990)Lake Guaíba, Porto Alegre, state of Rio Grande do SulFortes & Hofmann (1999)Moenkhausiadoceana(Steindachner, 1877)state of Espírito SantoKohn et al. (1990)Oligosarcushepsetus(Cuvier, 1829)*Lake Guaíba, Porto Alegre, state of Rio Grande do SulFortes & Hofmann (1995)Oligosarcus robustusMenezes, 1969Lake Guaíba, Porto Alegre, state of Rio Grande do SulKohn et al. (1990); Fortes & Hofmann (1995)BryconidaeSalminusbrasiliensis(Cuvier, 1816)Rio Moji-Guaçu, Pirassununga, state of São PauloKohn et al. (1990)*see remarks in the text.
image/svg+xml223Genarchella parvain fsh from southern BrazilNeotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023Fortes & Hofmann (1999) reported G. parvain P. af. fasciatusfrom Lake Guaíba, but the authors did not provide the number of fsh specimens examined, the morphometry of the helminths, and the parameters of the infections. As for the genus Astyanax, this is the frst report of G. parvain A. henseliand A.lacustrisfrom southern Brazil, including data on morphometry and parameters of the infections in diferent host species. Also, we report a new locality (Itapeva Lagoon) for G. parva, contributing to the knowledge and distribution of the biodiversity of parasites in freshwater fshes.ACKNOWLEDGMENTSWe thank fsherman for host collection and feld assistance; the Buccal Pathology Laboratory (Faculty of Odontology), PUCRS for permission to use the microscope to take the photomicrographs; MG thanks the Brazilian National Research Council (CNPq) for a Ph.D. scholarship granted (process #140639/2016-0).Author contributions: CRediT (Contributor Roles Taxonomy)MG=Moisés Gallas LRPU=Laura Roberta Pinto UtzConceptualization: MGData curation: MGFormal Analysis: MGFunding acquisition: MG, LRPUInvestigation: MGMethodology: MGProject administration: MG, LRPUResources: MG, LRPUSoftware: MG, LRPUSupervision: MG, LRPUValidation: MG, LRPUVisualization: MG, LRPUWriting – original draft: MG, LRPUWriting – review & editing: MG, LRPUBIBLIOGRAPHIC REFERENCESBertaco, V.A., & Lucena, C.A.S. (2010). Redescription of Astyanax obscurus(Hensel, 1870) and A. laticeps(Cope, 1894) (Teleostei: Characidae): two valid freshwater species originally described from rivers of Southern Brazil. Neotropical Ichthyology,8, 7-20.Buckup, P.A., Menezes, N.A., & Ghazzi, M.S. (eds). (2007). Catálogo das espécies de peixes de água doce do Brasil. Museu Nacional.Bush, A.O., Laferty, K.D., Lotz, J.M., & Shostak, A.W. (1997). Parasitology meets ecology on its own terms: Margolis et al. revisited. Journal of Parasitology,83, 575-583.Ditrich, O., Scholz, T., Aguirre-Macedo, L., & Vargas-Vázquez, J. (1997). Larval stages of trematodes from freshwater molluscs of the Yucatán Peninsula, Mexico. Folia parasitologica,44, 109-127.Fortes, E., & Hofmann, R.P. (1985). Contribuição ao conhecimento dos parasitos de peixes do estuário do Guaiba, no Estado do Rio Grande do Sul. IV – Trematódeos digenéticos. Revista Brasileira de Medicina Veterinária,7, 209-210.Fortes, E., & Hofmann, R.P. (1995). Trematódeos gastrintestinais de Astyanax fasciatus(Cuvier, 1819), lambaris do lago Guaíba, Porto Alegre, Rio Grande do Sul, Brasil. A Hora Veterinária, 18, 55-56.Gallas, M., & Utz, L.R.P. (2019a). First report of Dendrorchis retrobilobaVolonterio & Ponce de León, 2005 (Digenea, Gorgoderidae) in Astyanaxaf. fasciatus(Cuvier, 1819) (Characiformes, Characidae) from southern Brazil. Check List, 15, 357-361.Gallas, M., & Utz, L.R.P. (2019b). Quadrigyrus torquatuscystacants Van Cleave, 1920 (Acanthocephala: Quadrigyridae) parasitizing species of Astyanax(Characiformes: Characidae) from southern Brazil. Biota Neotropica, 19, e20180693.
image/svg+xml224Neotropical Helminthology (Lima). Vol. 17, Nº2, jul - dic 2023Gallas et al.Gallas, M., & Utz, L.R.P. (2019c). Revalidation of Saccocoelioides bacilliformis(Digenea, Haploporidae) parasitizing species of Astyanax(Characiformes, Characidae) from southern Brazil. Iheringia. Serie Zoologia, 109, e2019039.Kloss, G.R. (1966). Helmintos parasitas de espécies simpátricas de Astyanax(Pisces, Characidae). Papéis Avulsos de Zoologia, 18, 189-219.Kohn, A., & Fernandes, B.M.M. (1988). Revision of the Brazilian species of the genus HalipegusLooss, 1899 (Trematoda: Derogenidae). Systematic Parasitology, 11, 129-137.Kohn, A., Fernandes, B.M.M., Gibson, D.I., & Fróes, O.M. (1990). On the Brazilian species of halipegine genera (Trematoda: Derogenidae) from fshes, with new morphological data, hosts and synonyms. Systematic Parasitology, 16, 201-211.Kohn, A., Fernandes, B.M.M., & Cohen, S.C. (2007). South American Trematodes Parasites of Fishes. Imprinta Express.Kohn, A., Moravec, F., Cohen, S.C., Canzi, C., Takemoto, R.M., & Fernandes, B.M.M. (2011). Helminths of freshwater fshes in the reservoir of the Hydroelectric Power Station of Itaipu, Paraná, Brazil. Check List, 7, 681-690.Lucena, C.A.S., & Soares, H.G. (2016). Review of species of the Astyanax bimaculatus“caudal peduncle spot” subgroup sensuGarutti & Langeani (Characiformes, Characidae) from the rio La Plata and rio São Francisco drainages and coastal systems of southern Brazil and Uruguay. Zootaxa, 4072, 101-125.Lucena, C.A.S., Castro, J.B., & Bertaco, V.A. (2013). Tree new species of Astyanaxfrom drainagens of southern Brazil (Characiformes: Characidae). Neotropical Ichthyology, 11, 537-552.Lunaschi, L.I. (1990). Helmintos parasitos de peces de agua dulce de la Argentina IX. El género GenarchellaTravassos, Artigas y Pereira, 1928, y descripción de G. parvaTravassos, Artigas y Pereira, 1928, y G. fragilissp. nov. (Trematoda, Derogenidae). Studies on Neotropical Fauna and Environment, 25, 125-132.Scholz, T., Vargas-Vazquez, J., & Salgado-Maldonado, G. (1995). Revision of Genarchellaspecies (Digenea: Derogenidae) parasitizing freshwater fshes in Mexico and Central America. Journal of Natural History, 29, 1403-1417.Terán, G.E., Benitez, M.F., & Mirande, J.M. (2020). Opening the Trojan horse: phylogeny of Astyanax, two new genera and resurrection of Psalidodon(Teleostei: Characidae). Zoological Journal of the Linnean Society, 190, 1217-1234.Travassos, L., Artigas, P., & Pereira, C. (1928). Fauna helminthologica dos peixes de água doce do Brasil. Archivos do Instituto Biológico, 1, 5-68.Vilella, F.S., Becker, F.G., & Hartz, S.M. (2002). Diet of Astyanaxspecies (Teleostei, Characidae) in an Atlantic Forest River in Southern Brazil. Brazilian Archives of Biology and Technology, 45, 223-232.Virgilio, L.R., Martins, W.M.O., Lima, F.S., Takemoto, R.M., Camargo, L.M.A., & Meneguetti, D.U.O. (2022). Endoparasite fauna of freshwater fsh from the upper Juruá River in the Western Amazon, Brazil. Journal of Helminthology, 96, e55.Yamaguti, S. (1975). A synoptical review of life histories of digenetic trematodes of vertebrates with special reference to the morphology of their life cycles. Keigaku Publishing Co.Received October 1, 2023.Accepted December 5, 2023.