Abstract

ORIGINAL ARTICLE / ARTÍCULO ORIGINAL

Resumen

A NEW SPECIES OF TEMNOCEPHALA BLANCHARD (PLATYHELMINTHES,

TEMNOCEPHALIDA) ECTOSYMBIONT ON DILOCARCINUS SEPTEMDENTATUS (DECAPODA,

TRICHODACTYLIDAE) FROM THE BRAZILIAN AMAZONIA

NUEVA ESPECIE DE TEMNOCEPHALA BLANCHARD (PLATYHELMINTHES,

TEMNOCEPHALIDA) ECTOSIMBIONTE EN DILOCARCINUS SEPTEMDENTATUS (DECAPODA,

TRICHODACTYLIDAE) DE LA AMAZONÍA BRASILERA

1* 1 1**

Samantha A. Seixas , José F. R. Amato & Suzana B. Amato

1 Departamento de Zoologia, Instituto de Biociências, Universidade Federal do Rio Grande do Sul. Caixa Postal 15014,

91501-970 Porto Alegre, Rio Grande do Sul, Brasil. E-mail: samantha_bio@yahoo.com.br; josefelipeamato@gmail.com;

sbamato@ufrgs.br

*CAPES Doctoral Fellow. **CNPq Productivity Scholar.

Suggested citation: Seixas, S.A., Amato, J.F.R. & Amato, S.B. 2011. A new species of Temnocephala Blanchard

(Platyhelminthes, Temnocephalida) ectosymbiont on Dilocarcinus septemdentatus (Decapoda, Trichodactylidae) from the

Brazilian Amazonia. Neotropical Helminthology, vol 5, nº 2, pp. 201-212.

Key words: crustaceans-Neotropic Region-taxonomy-temnocephalids

Temnocephala longivaginata sp. n. ectosimbionte en Dilocarcinus septemdentatus (Herbst, 1783), es

descrita del Estado de Pará, Amazonia brasilera. Cincuenta cangrejos fueron colectados en Rio Peixe-

Boi, Municipio de Peixe-Boi. Dilocarcinus septemdentatus es el hospedero-tipo de Temnocephala

microdactyla Monticelli, 1903, especie con una descripción original pobre, pero que fue re-descrita en

otras dos ocasiones en diferentes hospederos: Sylviocarcinus australis Magalhães & Türkay, 1996 y

Dilocarcinus pagei Stimpson, 1861. La nueva especie es más parecida con Temnocephala

pignalberiae Dioni, 1967, de quien se diferencia por los siguientes caracteres: 1. cirro con un círculo de

escleritos en la porción distal del introverto, seguida por una porción lisa (sin espinas o cristas); 2.

porción proximal interna del introverto con cristas longitudinales; 3. vesicula 'intermedia' larga,

sustituyendo los receptáculos seminales; 4. vagina larga, con una extensión en la porción distal,

próximo al esfínter vaginal asimétrico; y 5. placas dorsolaterales, sincitiales 'excretoras' pequeñas y en

forma de 'suela de zapato'. Hasta el momento, todas las especies de Temnocephala tienen el introverto

del cirro liso o con espinas. Cristas en la pared interna del introverto y un círculo de escleritos son

registrados por primera vez en Temnocephalidae.

Palabras clave: crustáceos-Región Neotropica-taxonomía-temnocefalideos

Neotrop. Helminthol., 5(2), 2011

2011 Asociación Peruana de Helmintología e Invertebrados Afines (APHIA)

Versión Impresa: ISSN 2218-6425 / Versión Electrónica: ISSN 1995-1043

Temnocephala longivaginata sp. n., an ectosymbiont on Dilocarcinus septemdentatus (Herbst, 1783),

is described from crabs in the State of Pará, Brazilian Amazonia. Fifty crabs were collected from the Rio

Peixe-Boi, Municipality of Peixe-Boi. Dilocarcinus septemdentatus is the type host of Temnocephala

microdactyla Monticelli, 1903, a species with a precarious original description, but redescribed on two

other occasions from different hosts: Sylviocarcinus australis Magalhães & Türkay, 1996 and

Dilocarcinus pagei Stimpson, 1861. The new species is most similar to Temnocephala pignalberiae

Dioni, 1967, but differs by having the following characters: 1. cirrus with a circle of sclerites in the

distal portion of the introvert, followed by a smooth portion (without spines or ridges); 2. proximal,

inner portion of the introvert with longitudinal ridges; 3. vesicula 'intermedia' long, replacing the

seminal receptacles; 4. vagina long, with a widening of its distal portion, near the asymmetrical vaginal

sphincter; and 5. small and 'shoe sole' shaped dorsolateral 'excretory' syncytial epidermal plates. Prior

to the present study, all species of Temnocephala were shown to have the cirrus' introvert either smooth

or with spines. Ridges in the inner wall of the introvert and a circle of sclerites are here recorded for the

first time in Temnocephalidae.

201

INTRODUCTION

Crustacea Brünnich, 1772 is the most common host

group for species of Temnocephala Blanchard,

1849-of the 27 species described from the

Neotropic Region, 17 are ectosymbionts on

crustaceans. Dilocarcinus septemdentatus (Herbst,

1783) (Trichodactylidae) (Figs 1-2) is the type host

of Temnocephala microdactyla Monticelli, 1903

collected in Carandazinho, State of Mato Grosso,

Brazil (Monticelli, 1903). Pereira & Cuocolo

(1941) redescribed the species due to the

precariousness of the original description,

especially that of the cirrus. These authors based

their redescription of T. microdactyla on specimens

collected in Bodoquena, MS, from Trichodactylus

pictus (= Sylviocarcinus australis Magalhães &

Türkay, 1996). Based on the work of Pereira &

Cuocolo (1941), Dioni (1967) identified three

species (T. microdactyla, Temnocephala

santafesina Dioni, 1967, and Temnocephala

pignalberiae Dioni, 1967) from trichodactylidean

crustaceans in Argentina. Dioni (1967) did not

indicate the type host of T. pignalberiae, which

could be Dilocarcinus pagei Stimpson, 1861, S.

australis, or an unidentified species of

Trichodactylus Latreille, 1828 from three different

localities in Argentina. Damborenea (1992)

recorded T. pignalberiae on D. pagei and

Sylviocarcinus pictus (Milne-Edwards, 1853) also

in Argentina. Recently, T. pignalberiae had its

description updated by Amato et al. (2010) based

on ectocommensal specimens found on D. pagei

collected in the States of São Paulo (SP) and Mato

Grosso (MT). This was the first record for the

species in Brazil. The present paper adds one more

ectocommensal species of Temnocephala living on

trichodactylids, and presents a complete

documentation study.

MATERIAL AND METHODS

Fifty crabs were collected from Rio Peixe-Boi

o o

(01 07'17.65”S, 47 18'48.35”W), Municipality of

Peixe-Boi, State of Pará (PA), Brazil. Specimens of

D. septemdentatus were collected manually or with

dip nets by Edilson R. Mattos and were taken still

alive to the “Laboratório de Pesquisa Carlos

Azevedo – LPCA, Universidade Federal Rural da

A m a z ô n i a (U F R A ) ” , i n B e l é m , PA .

Temnocephalans were fixed with 10% phosphate-

buffered formalin 90ºC or with AFA (70 GL

ethanol; formalin 37%; glacial acetic acid), under

slight cover slip pressure, following the protocols

established by Amato et al. (2007) and Seixas et al.

(2010). The specimens were sent to the

“Laboratório de Helmintologia, Universidade

Federal do Rio Grande do Sul (UFRGS)”, where

some specimens were stained in Delafield's

hematoxylin or aceto-carmine /fast green, cleared

in cedar oil and mounted as permanent slides in

Canada balsam. Some specimens were prepared

for the Scanning Electron Microscopy (SEM) at the

“Centro de Microscopia Eletrônica da UFRGS

(CME)”. The temnocephalans from D.

septemdentatus were studied through a series of

techniques fully described by Amato et al. (2007)

and Seixas et al. (2010), focusing especially on the:

1. morphology of the cirrus structure; 2.

morphology of the vagina and other female

reproductive organs; 3. distribution of the

rhabditogenic glands in juveniles; and, 4. paired,

dorsolateral, post-tentacular 'excretory' syncytial

plates (DLSPs). Photomicrographs were taken

with the microscopes Zeiss Axiolab and Leica

DMR Hc equipped with Nomarski´s differential

interference contrast prisms (DIC). The

photographic images and line drawings were

scanned and prepared using CorelDraw X5 and

Adobe's Photoshop CS2, respectively.

Measurements are in micrometers (μm) unless

otherwise indicated and were taken from

specimens killed under slight cover slip pressure

and mounted in Canada balsam; ranges are

followed (between parentheses) by the mean, the

standard deviation values, and the number of

specimens measured for a given character (when

different than 11). Cirrus measurements were taken

from extracted cirri mounted in Faure´s mounting

medium (F), while the terminology used to

describe the male reproductive structures followed

Seixas et al. (2010). The whole mounts of adult and

juvenile type specimens, as well as slides

containing individual cirri mounted in (F) were

deposited in the following scientific collections: 1.

“Coleção Helmintológica do Instituto Oswaldo

Cruz” (CHIOC), Rio de Janeiro, RJ, Brazil; 2.

“Colección de Invertebrados, División Zoología

Invertebrados, Museo de La Plata (MLP)”, La

Plata, Argentina; and 3. “Coleção Helmintológica

do Laboratório de Helmintologia, Departamento

de Zoologia, Universidade Federal do Rio Grande

do Sul”, Porto Alegre, RS.

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New species of Temnocephala from the Brazilian Amazonia Seixas et al.

RESULTS

Description. Based on 20 specimens collected: 11

whole mounted adults; 1 juvenile; 3 specimens

mounted on stubs for SEM; 5 dissected cirri

mounted in (F); 11 specimens measured.

External characteristics. Body (without tentacles)

(Figs 5 and 22) 2.48–4.54 mm (3.14 mm ± 678)

long, 1.61–2.79 mm (2.02 mm ± 419) wide;

adhesive disk ventral, subterminal, completely

covered by body (Figs 5 and 22) 501–905 (651 ±

124) long, 529–836 (663 ± 102) wide; disc

peduncle 390–613 (481 ± 90) wide. Red

pigmentation of the eyes not observed. Two

dorsolateral, epidermal 'excretory' syncytial plates

(DLSP ) small, 'shoe sole-shaped (Figs 11-12), left

plate 152.7–313.2 (230 ± 80; 3) long, 53.7–119.5

(83 ± 34; 3) wide; right plate 165.6–310.2 (229 ±

74; 3) long, 65.7–84.6 (76 ± 10; 3) wide; length

ratio of DLSPs /total body length, without

tentacles, 14: 1. Excretory pore in the equatorial

line of the plate, near the internal limit (Fig. 12).

Glands. Rhabditogenic glands forming bunches

(average 15 cells), in lateral fields of body (Figs 6-

7), 33-100 (64 ± 15) in diameter, ducts conspicuous

(Figs 7 and 23). Two groups of five Haswell glands,

showing little affinity with hematoxylin, in front of

the brain transverse band, diameter of largest cell

17–39 (24 ± 13; 3). Disc glands between adhesive

disc and genital complex, 22–61 (40 ± 11) in

diameter, forming two, lateral bunches extending

from posterior testes to margin of adhesive disc

(Figs 6-7), including pair, of large, round, more

central cells (paranephrocytes?), 67–155 (111 ±

27) long (Figs 6-7).

Reproductive system. Female. Ovary located

between vagina and vesicula resorbens, 88–190

(144 ± 40; 9) long, 75–170 (113 ± 34; 9) wide (Figs

9 and 25). Vitellarium arborescent, wispy, barely

covering intestine dorsally (Figs 8 and 22); vagina

long, 56–210 (147 ± 49; 8) long, 60-80 (72 ± 8; 8)

wide (Figs 9 and 25) with a widening of its distal

portion; vaginal sphincter asymmetrical, 75-97.5

(83.5 ± 6; 10) in total diameter (Figs 9 and 25);

diameter of anterior portion 30-37.5 (33 ± 4; 10),

diameter of posterior portion 32.5-42.5 (38 ± 2;

10); vesicula 'intermedia' 40-80 (60 ± 16; 5) long;

vesicula resorbens usually full of sperm (Fig. 25),

102.5–360 (227 ± 101; 6) long; 130–310 (264 ± 97;

6) wide; wall thickness 5–30 (16 ± 9; 6).

Temnocephala longivaginata n. sp.

(Fig. 3 - 25) Male. Four testes quite small, usually rounded,

slightly oblique; deferent vessels unite in large,

pyriform, thick-walled seminal vesicle, 215–350

(288 ± 38) long, 40–100 (70 ± 18) wide; wall

thickness 5–10 (9 ± 2); prostatic bulb elongated,

with thick muscular walls, 100–340 (239 ± 63)

long, 87.5–190 (111 ± 24) wide, wall thickness

5–20 (11 ± 5) (Fig. 10). Cirrus 112.5–130 (121.25 ±

12.4; 2) long, with a circle of sclerites (average 10)

in the distal portion of the introvert (Figs 13 and

20), followed by a smooth portion (without spines

or ridges) (Figs 14 and 21). Introvert proximal

portion with longitudinal ridges reaching the

proximal limit (Figs 14-19, and 21). Shaft 82.5–95

(88.8 ± 8.9; 2) long, shaft maximum width at base

57.5–62.5 (60 ± 3; 2) (Fig. 21); introvert 30 (n = 2)

long; introvert width at base 20 (n = 2); maximum

introvert width at level of swelling 22.5 (n = 2).

Introvert´s swelling with approximately 10 ridges

(Figs 14-19). Ratio between total body length,

without tentacles/ total length of cirrus 26: 1; ratio

between total length of cirrus/ maximum width of

shaft´s base 2: 1; ratio between total length of

cirrus/ total length of introvert 4: 1.

Taxonomic summary.

Type host: Dilocarcinus septemdentatus (Herbst,

1783) (Trichodactylidae).

Type locality: Rio Peixe-Boi (01º07'17.65”S,

47º18'48.35”W), Municipality of Peixe-Boi, PA,

Brazil.

Site of infestation: branchial chambers.

Helminth specimens deposited: “Coleção

Helmintológica do Instituto Oswaldo Cruz”:

CHIOC 37458a - Holotype (SBA 3226-1-3);

CHIOC 37458b - juvenile paratype (SBA 3226-1-

7); CHIOC 37459 - cirrus in Faure´s mounting

medium (SBA 3227-1-6). “Colección de

Invertebrados, División Zoología Invertebrados,

Museo de La Plata”: MLP 6292 - paratype (SBA

3226-1-1); - cirrus in Faure´s mounting medium

(SBA 3227-1-7A).

Other helminth specimens examined:

Temnocephala pignalberiae – Amato et al. 2010:

CHIOC Nº 37308; CHIOC Nº 37309; CHIOC Nº

37310 a-b; CHIOC Nº 37311; MLP Nº 6091; MLP

Nº 6092; MLP Nº 6094; MLP Nº 6095. “Coleção de

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Neotrop. Helminthol., 5(2), 2011

Invertebrados do Instituto Nacional de Pesquisas

da Amazônia (INPA)”: INPA Nº 525; INPA Nº 526;

INPA Nº 527; INPA Nº 528. Temnocephala

pignalberiae – Damborenea (1992):

MLP Nº 3126; MLP Nº 3127.

Host specimens deposited: The collector of the

crabs from Rio Peixe-Boi for the present work,

Edilson R. Matos, did not deposit specimens, but in

1994 deposited two male specimens collected from

the same location in the collection of the “Museu de

Zoologia da Universidade de São Paulo (MZUSP)”

under the Nº 11694, which were identified by

Gustavo A. S. de Melo as D. septemdentatus.

Etymology: the specific epithet is given after the

unusually long vagina of this species.

DISCUSSION

Pereira & Cuocolo (1941) when redescribing T.

microdactyla assumed that the large specimens

with long cirrus without introvert spines collected

at Bodoquena, MS belonged to the same species

described by Monticelli (1903). The original

description mentioned the short size of the

tentacles in relation to body size, thus explaining

the specific epithet. Since the reproductive system

on the original description of T. microdactyla was

not described, it is possible that this species might

be as described by Pereira & Cuocolo (1941), by

Dioni (1967), and by Damborenea (1992), but it

also could be T. pignalberiae, recently collected in

Poconé, MT, less than 20 km from the type locality

of T. microdactyla by Amato et al. (2010) or the

Célio Magalhães (pers. comm.) received dorsal

and ventral pictures of a female crab used in the

present work, but indicated that the taxonomy

within the genus Dilocarcinus is done exclusively

through the male gonopods and recommended the

utilization of the name D. septemdentatus for these

crabs. He also cautioned that Goyazana castelnaui

(H. Milne Edwards, 1853) may occur in the region,

but has not yet been recorded at Rio Peixe-Boi.

Magalhães & Türkay (2008) examined the male

specimens of D. septemdentatus deposited at the

MZUSP, and confirmed that they, in fact, were D.

septemdentatus.

species being described in the present work found

in the type host of T. microdactyla.

The ectocommensal temnocephalans found on D.

septemdentatus are most similar to T. pignalberiae

in size and shape of the body, but show important

differences in the reproductive system (Figs 9-10,

and 25). The new species differs from T.

pignalberiae by having the cirrus internal wall with

ridges and unique sclerites in the distal portion of

the introvert, followed by a smooth portion

(without spines or ridges) (Figs 13-21). To present,

all species of Temnocephala have the cirrus'

introvert either with spines or are smooth, i.e.,

without spines. Ridges in the inner wall of the

introvert were recorded for the first time in

Temnocephalidae. The cirrus of T. pignalberiae is

similar in size (105µm in length), but lacks either

spines or ridges. It also differs by having a long

vesicula 'intermedia' (average of 60µm in length)

(Figs 9 and 25) replacing the seminal receptacles

and a long vagina (average 147µm in length) (Figs

9 and 25) with a widening of its distal portion (Fig.

25), near the asymmetrical vaginal sphincter (Figs

9 and 25). The 'excretory' syncytial plates (DLSP )

also differ in shape from that of T. pignalberiae

which is large and elongate (Amato et al. 2010,

Figs 19-21, pg. 23). The new species has the

'excretory' syncytial plates (DLSP ) small and

'shoe sole'-shaped (Figs 11-12).

The finding of characters so unusual as a circle of

sclerites and ridges in the introvert of T.

longivaginata n. sp., a species close to T.

pignalberiae which has the introvert smooth and

occurs in another species of Dilocarcinus calls

attention to the fact that temnocephalans cannot be

studied by the techniques used prior to 1994, which

used specimens collected from hosts fixed in cold

ethanol and studied only through pressure between

slide and coverslip. Without the extraction of the

cirrus to be mounted in F, from specimens collected

alive fixed with hot formalin and observed with

DIC and SEM one would not have found the fine

details of the introvert shown in the present work.

This allowed the best comparison possible between

the shape of the DLSPs of the new species and

those of T. pignalberiae. In our opinion species of

Temnocephala described prior to the year 2000

should be collected again from live hosts and

reexamined with the techniques used in the present

work.

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New species of Temnocephala from the Brazilian Amazonia Seixas et al.

Figures 1–3. Female specimen of Dilocarcinus septemdentatus. 1. dorsal view. 2. ventral view. Scale bars = 3 cm. 3. live eggs

deposited internally in the upper side of the carapace. Scale bar = 500 µm. Figures 4–5. Temnocephala longivaginata n. sp. 4.

young specimen. Scale bar = 1 mm. 5. Adult specimen. Scale bar = 500 µm.

205

Neotrop. Helminthol., 5(2), 2011

Figure 6. Temnocephala longivaginata n. sp. Juvenile, ventral view, showing rhabditogen glands (rg), esophageal glands (eg),

posterior disc glands (dg), incipient cirrus (c), and the pair of large disk glands (paranephrocytes?) (head arrows). Scale bar = 150

µm.

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New species of Temnocephala from the Brazilian Amazonia Seixas et al.

Figures 7-10. Temnocephala longivaginata n. sp. 7.

posterior disc glands and the pair of large disk glands

(paranephrocytes?) (head arrows). Scale bar = 100 µm. 8. Incomplete diagram of an adult specimen, ventral view, showing:

adhesive disk (ad), anterior testis (at), excretory vesicles (ev), mouth (m), pharynx (ph), posterior testis (pt),

tentacles (t), and vitelline glands (vg). Scale bar = 500 µm. 9. Female reproductive organs: anterior portion of the vaginal sphincter

(avs), genital atrium (ga), genital pore (gp), ovary (ov), vagina (va), vitelline glands duct (vgd), vesicula 'intermedia' (vi), vesicula

resorbens (vr), and posterior portion of the vaginal sphincter (pvs). Scale bar = 100 μm. 10. Male reproductive system: cirrus (c),

opening in the prostatic bulb wall (head arrow), prostatic bulb (pb), prostatic cells (pc), prostatic secretion (ps), prostatic vesicle

(pv), seminal vesicle (sv), and vasa deferentia (vd). Scale bar = 100 μm.

Juvenile, ventral view, showing rhabditogenic glands (rg) extending along

sides of intestinal sac, and its ducts (rgd) entering tentacles. The (dg)

intestinal sac (i),

207

Neotrop. Helminthol., 5(2), 2011

Figures 11–12. Temnocephala longivaginata n. sp. observed with SEM. 11. Entire specimen, showing the dorsolateral 'excretory'

syncytial plates (head arrows) and position of excretory pores (n – white arrows). Scale bar = 500 μm. 12. Left DLSP (head arrows)

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New species of Temnocephala from the Brazilian Amazonia Seixas et al.

Figures 13–19. Temnocephala longivaginata n. sp. cirrus mounted in (F) and observed with DIC. 13. En face view of the distal

portion of the introvert indicating the circle of sclerites (*) and the cirrus lumen (cl). Scale bar = 10 μm. 14. Cirrus, showing the

proximal limit of the introvert (black head arrows) and the smooth portion (*) between the ridges and the sclerites. 15-19. Cirrus

introvert observed in different focusing planes. Scale bar = 20 μm.

209

Neotrop. Helminthol., 5(2), 2011

Figures 20–21. Temnocephala longivaginata n. sp. 20. En face line drawing of distal portion of the introvert indicating the circle

of sclerites (*) and the cirrus lumen (cl). Scale bar = 5 μm. 21. Line drawing of cirrus, showing the sclerites portion of the introvert

(sp), the smooth portion (smp), and the ridges (r) of the inner wall of the introvert, just after the limit between the introvert and the

shaft (head arrow). Scale bar = 10 μm.

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New species of Temnocephala from the Brazilian Amazonia Seixas et al.

Figures 22–25. Temnocephala longivaginata n. sp. 22. Adult specimen pressed between slide and cover slip, showing that too

much pressure may be good to allow all characters to be observed in the same focusing plane, but alters the body shape. Scale bar =

250 μm. 23. Pair of largest cells of Haswell glands (head arrows) and the . Scale bar

= 200 μm. 24. Mouth (m), mouth sphincter (ms), and pharyngeal sphincter (ps). Scale bar = 200 μm. 25. Female reproductive

system: anterior portion of the vaginal sphincter (avs), genital atrium (ga), ovary (ov), posterior portion of the vaginal sphincter

(pvs), vagina (va), vesicula 'intermedia' (arrow); widening of the vagina (head arrows), and vesicula resorbens (vr). Scale bar =

100 μm.

ducts of rhabditogenic glands entering tentacles

211

Neotrop. Helminthol., 5(2), 2011

ACKNOWLEDGEMENTS

Special thanks are due to Jorge Ernesto de Araújo

Mariath and Rinaldo Pires dos Santos, Laboratório

de Anatomia Vegetal, Instituto de Biociências,

UFRGS, for the permission to use the Leica DMR

Hc microscope to make the DIC

photomicrographs. We are indebted to Edilson

Rodrigues Matos and his students Marcela,

Michelle and Erika, at the “Laboratório de

Pesquisa Carlos Azevedo – LPCA, Universidade

Federal Rural da Amazônia (UFRA)” for the

collection of the crab hosts and the

temnocephalans, respectively. Special thanks are

also due to the staff of the “Centro de Microscopia

Eletrônica”, UFRGS for the SEM operation; to

Maria Cristina Damborenea, Curator, Invertebrate

Collection at Museo de La Plata (MLP), for the

loan of some Argentinean specimens deposited at

the Museo de La Plata; to CAPES (Coordenação de

Aperfeiçoamento do Pessoal de Nível Superior) for

the Doctoral Scholarship awarded to SAS; to Abner

Chiquieri for his contribution to the correct

formation of the specific epithet of the new species;

to Bibiana Oliveira for her help in the laboratory;

and, especially, to Philip J. Scholl, for kindly

reviewing the English of the several versions of the

manuscript.

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Instituto Biológico, vol. 12, pp. 101-127.

Redescription of Temnocephala iheringi

(Platyhelminthes: Temnocephalida) based

on specimens from Pomacea canaliculata

(Mollusca: Ampullariidae) of the state of

Rio Grande do Sul, Brazil: the possible

type host and type locality. Zoologia, vol.

27, pp. 245-257.

Correspondence to author/Autor para correspondencia:

Samantha A. Seixas

Departamento de Zoologia, Instituto de Biociências,

Universidade Federal do Rio Grande do Sul. Caixa Postal

15014, 91501-970 Porto Alegre, Rio Grande do Sul, Brasil.

E-mail/correo electrónico:

samantha_bio@yahoo.com.br

Received July 12, 2011.

Accepted August 30, 2011.

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New species of Temnocephala from the Brazilian Amazonia Seixas et al.