image/svg+xmlISSN Versión impresa 2218-6425ISSN Versión Electrónica 1995-1043REVIEW ARTICLE / ARTICULO DE REVISIÓN CHECKLIST OF HELMINTHS ASSOCIATED WITH CONTINENTAL TESTUDINES FROM SOUTH AMERICA LISTA DE HELMINTOS ASOCIADOS A LOS TESTUDINES CONTINENTALES DE AMÉRICA DEL SUR1Laboratório de Parasitologia de Animais Silvestres (LAPASIL), Departamento de Microbiologia e Parasitologia, Instituto de Biologia, Universidade Federal de Pelotas (UFPel), Caixa Postal: 354, CEP 96010-900, Pelotas, RS, Brazil.*Corresponding author: phrybio@hotmail.comCarolina S. Mascarenhas: https://orcid.org/0000-0002-7045-0926 Gertrud Müller: https://orcid.org/0000-0002-2213-672111Carolina S. Mascarenhas* & Gertrud MüllerNeotropical Helminthology97doi:10.24039/rnh20201511047Neotropical Helminthology, 2021, 15(1), ene-jun:97-126.DABSTRACTThis study collected records of 135 taxaof parasitic helminths (Nematoda, Trematoda, Cestoda, Monogenoidea and Acanthocephala) and ecto-symbionts (Temnocephalida) associated with continental Testudines from South America. Eighty-nine helminths were identified at the species level while others were identified up to genus or family levels. The greatest diversity of helminths associated with Testudines was reported in Brazil. Chelidae was the family with the largest number of helminth species. Regarding the conservation status, 17 Testudines species with helminth records are cited in the IUCN Red List of Threatened Species. Knowledge of helminth biodiversity and of relations between these organisms and Testudines can provide important data on host biology. Therefore, information generated by studies of helminths can contribute to research which aims at the conservation of organisms and their habitats.DKeywords: Acanthocephala – biodiversity – Cestoda – Chelidae – conservation – Digenea –ecto-symbiont – Emydidae – freshwater turtle – Geoemydidae – IUCN – Kinosternidae – Monogenoidea – Nematoda – parasite – Podocnemididae – Temnocephala – Testudinidae – tortoise
image/svg+xmlAmong helminths that can be associated with Testudines, there are parasites (e.g., Nematoda, Trematoda) (Vicente et al., 1993; Fernandes & Kohn, 2014) and ectosimbionts (e.g., Temnocephalida) (Martínez-Aquinoet al., 2014). Parasitic organisms represent a significant part of biodiversity; according to Windsor (1998), they represent the largest number of species on Earth. There is at least a kind of parasite associated with every free-living species (Poulin, 1999). According to Dobson et al.(2008), there are between 75,000 and 300,000 species of helminths parasitizing vertebrates; this estimate suggests that there may be more parasitic species than free-living ones, reinforcing Windsor's findings (1998). Temnocephalida are composed of freshwater ectosymbionts with specificity to the host group or species (Martínez-Aquino et al., 2014).Testudines comprise 478 taxa(356 species and 122 subspecies) worldwide; seven species and three subspecies, i. e., 10 taxa(2.1%), have been extinct (Rhodin et al., 2017). In South America, continental Testudines are represented by 71 taxa; 37 (35 threatened ones and two extinct ones) out of 71 taxaare cited in the International Union for Conservation of Nature (IUCN) Red List of Threatened Species (Rhodin et al., 2017; IUCN, 98RESUMENEn este estudio se recogieron registros de 135 taxonesde helmintos parásitos (Nematoda, Trematoda, Cestoda, Monogenoidea y Acanthocephala) y ectosimbiontes (Temnocephalida) asociados a Testudines continentales de Sudamérica. Se identificaron 89 helmintos a nivel de especie, mientras que otros se identificaron hasta el nivel de género o familia. La mayor diversidad de helmintos asociados a los Testudines se registró en Brasil. Chelidae fue la familia con el mayor número de especies de helmintos. En cuanto al estado de conservación, 17 especies de Testudines con registros de helmintos están citadas en la Lista Roja de Especies Amenazadas de la IUCN. El conocimiento de la biodiversidad de los helmintos y de las relaciones entre estos organismos y los Testudines puede aportar datos importantes sobre la biología de los hospedadores. Por lo tanto, la información generada por los estudios de los helmintos puede contribuir a la investigación que tiene como objetivo la conservación de los organismos y sus hábitats.INTRODUCTION2020). Continental Testudines undergo several negative impacts that affect their populations. The main threats are: fragmentation of terrestrial or aquatic habitats, degradation of water quality, disorderly occupation of spawning grounds, consumption of meat and eggs, illegal trade for pets and death by running over (Gibbons et al., 2000; Gibbs & Shriver, 2002; Steen & Gibbs, 2004; Bujes & Verrastro, 2008). Anthropic actions, such as habitat change, loss of biodiversity, pollution, climate change and introduction of species, can also impact helminth fauna (Lafferty & Kuris, 2005). Dobson et al. (2008) estimated that from 3% to 5% of parasitic helminths will be threatened with extinction in the next 50 to 100 years. In the literature, there is no checklist of parasitic and ectosymbiont helminths associated with continental Testudines from South America. Cohen et al.(2013) carried out a checklist of Monogenoidea, while Fernandes & Kohn (2014) introduced one of Trematoda from South America and Martínez-Aquino et al.(2014) listed the records of Temnocephalida in the Neotropical region. Therefore, this checklist, which gathered the records of parasitic and ectosymbiont helminths associated with continental Testudines from South America, aiming at contributing to the knowledge of the diversity of helminths associated with the group and at encouraging helminthological studies with group of vertebrates. Neotropical Helminthology, 2021, 15(1), ene-junPalabras clave: Acanthocephala – biodiversidad – Cestoda – Chelidae – conservación – Digenea – ectosimbionte – Emydidae – Geoemydidae – IUCN – Kinosternidae – Monogenoidea – Nematoda parasito – Podocnemididae – Temnocephala – Testudinidae – tortuga de agua dulce Mascarenhas & Müller
image/svg+xml99The checklist resulted from information published in articles, books, theses and dissertations. Some specimens deposited in the Helminth Collection that belongs to the Parasitology Laboratory of Wild Animals (CHLAPASIL) at the Microbiology and Parasitology Department in the Institute of Biology at the Universidade Federal de Pelotas (UFPel), located in Pelotas, RS, Brazil, were also examined. Classification and systematization of helminths were carried out in agreement with Anderson et al.(2009) for Nematoda; Gibson et al.(2002), Jones et al.(2005), and Bray et al.(2008) for Digenea; Khalil et al.(1994) for Cestoda; Boeger & Kritsky (1993) for Monogenoidea; Petrochenko (1971) for Acanthocephala; and Tyler et al.(2006-2019) for Temnocephalida. Classification and systematization of hosts followed Rhodin et al.(2017).Helminth taxaare shown in alphabetical order in their respective superfamilies, followed by host taxa, site of infection (parasitic helminths) or infestation (ectosymbionts), geographical location and respective bibliographical references (between parentheses and chronological order). Citation of helminth and host species in this list does not mean that the authors agree with their validity and taxonomy. The list of host-helminth includes the conservation status of Testudines species in agreemen with the Red List of Threatened Species issued by the IUCN (2020).One hundred and thirty-five helminths were recorded in association with 39 continental Testudines (34 species, three identified at the genus level and two unidentified) from nine South American countries. Eighty-nine helminths (40 Trematoda, 38 Nematoda, six Monogenoidea, three Temnocephalida and two Cestoda) were identified at the species level, while the others were identified up to genus (44 taxa) and family levels (2 taxa).The greatest diversity of helminths associated with Testudines was reported in Brazil (94 taxa), MATERIAL AND METHODSfollowed by Uruguay (17 taxa), Peru (15 taxa), Colombia (14 taxa), Argentina (13 taxa), Venezuela (10 taxa), Ecuador (10 taxa), French Guiana (2 taxa), Paraguay (1 taxa) and Bolivia (1 taxa). Figure 1 shows the number of helminths (according to the group) registered in each country. No records of helminthological studies were found in Chile, Suriname and Guyana. Chelidae was not only the family with the greatest diversity of recorded species (32), but also the group with the largest number of hosts under study (12 species) (Fig. 2). Podocnemidae and Emydidae had records of 30 and 14 species of helminths, respectively (Fig. 2). The largest numbers of taxawere associated with Phrynops hilarii(Duméril & Bibron, 1835) (21 taxa) and Hydromedusa tectiferaCope, 1870 (20 taxa) (Chelidae) in records from Brazil, Uruguay and Argentina.Nematoda and Trematoda occurred in all groups of Testudines (Fig. 2); their main site of infection was the digestive tract of the hosts. Monogenoidea was mainly represented by PolystomoidesWard, 1917 species (Polystomatidae) parasites of the oral cavity of Chelidae species. Two Cestoda species, in Geoemydidae and in Chelidae, were recorded. Acanthocephala was only recorded in a Testudines species, Acanthochelys spixii(Duméril & Bibron, 1835) (Chelidae). Ectosymbiont helminths, Temnocephalidae, were recorded in six Chelidae species and in an Emydidae species. The digenetic trematode Nematophila grandis(Diesing, 1839) was the taxon recorded in the largest number of host species (17) belonging to Kinosternidae, Geoemydidae, Testudinidae, Chelidae and Podocnemidae. The species, which has wide geographical distribution, was found in Brazil, French Guyana, Venezuela, Ecuador, Peru, Paraguay and Argentina. Atractidae was the group of Nematoda with the largest number of representatives (27 taxa), which were mainly registered in Podocnemidae species in Brazil. Among Testudines reported by helminthological studies in South America, 17 were cited in the IUCN Red List of Threatened Species. However, three species, Kinosternon integrumLe Conte, 1854 (Kinosternidae), Emys orbicularis(Linnaeus, 1758) (Emydidae) and Kinixys erosa(Schweigger, 1812) (Testudinidae), are not native to the region. Figure 3 shows the number of helminths associated with species native to South America that were cited in the Red List. Most of these Testudines have RESULTSNeotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xmlFigure 2. Number of helminth species associated with continental Testudines families from South America. Numbers of species studied in each family are between parentheses.100Figure 1.Number of helminths associated with continental Testudines from South America.Neotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml101few records of helminths. However, Nematoda and Trematoda are the most frequently reported ones (Fig. 3).HELMINTH-HOST LISTPhylum Nematoda (Rudolphi, 1808)Superfamily Dioctophymatoidea Railliet, 1916Family Dioctophymatidae (Railliet, 1915)Dioctophyme Collet-Meygret, 1802Dioctophyme renale(Goeze, 1782) (larvae)Acanthochelys spixii (Duméril & Bibron, 1835), large intestine, BRAZIL (Rio Grande do Sul State) (Chaviel et al.,2020).Hydromedusa tectiferaCope, 1870, body cavity (surface of stomach, and muscles) and serous of stomach BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL 882).Phrynops hilarii (Duméril & Bibron, 1835), muscles, body cavity (surface of esophagus, stomach, lung, liver) and serous of stomach, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al., 2017).Trachemys dorbigni(Duméril & Bibron, 1835), body cavity, muscles, mesentery, stomach serous lining and on surfaces of the lung, heart, liver, pancreas, spleen and intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas & Müller, 2015a).Eustrongylides Jägerskiöld, 1909Eustrongylides sp. (larvae)Trachemys dorbigni(Duméril & Bibron, 1835), stomach (serous), BRAZIL (Rio Grande do Sul State) (Mascarenhas, 2014).Superfamily Strongyloidea Weinland, 1858Family Strongylidae Baird, 1853ChapiniellaYamaguti, 1961Chapiniella variabilis (Chapin, 1924)Chelonoidis carbonarius (Spix, 1824), large intestine, BRAZIL (Piauí State) (Freire et al.,2017; Freire et al., 2019).Chelonoidis denticulatus(Linnaeus, 1766),intestine, BRAZIL (Pará State) (Chapin, 1924).Figure 3. Number of registered helminths in association with native continental Testudines from South America cited in the IUCN Red List of Threatened Species (2020). LR – Lower Risk; NT – Near threatened; VU – Vulnerable; CR – Critically Endangered.Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml102Chelonoidis denticulatus(Linnaeus, 1766),large intestine, BRAZIL (Piauí State) (Freire et al.,2017; Freire et al., 2019).SauricolaChapin, 1924Sauricola sauricolaChapin, 1924Chelonoidis denticulatus(Linnaeus, 1766),intestine, BRAZIL (Pará State) (Chapin, 1924).Superfamily Oxyuroidea Railliet, 1916Family Pharyngodonidae Travassos, 1919 Thelandros Wedl, 1862Thelandros sp. Chelonoidis carbonarius (Spix, 1824), intestine,VENEZUELA (Bolivar State) (Pérez Mata et al., 2014).Pharyngodonidae gen. sp.Acanthochelys spixii (Duméril & Bibron, 1835), large intestine, BRAZIL (Rio Grande do Sul State) (present estudy, CHLAPASIL, 889).Superfamily Cosmocercoidea Skrjabin & Shikhobalova, 1951Family Kathlaniidae (Lane, 1914)Falcaustra Lane, 1915Falcaustra affinis(Leidy, 1856)Trachemys dorbigni(Duméril & Bibron, 1835), large intestine, heart, kidney, stomach, BRAZIL (Rio Grande do Sul State) (Mascarenhas & Müller, 2015b).Falcaustra tikasinghi(Schroeder, Schmidt & Everard, 1977)Rhinoclemmys annulata (Gray, 1860), digestive tract, ECUADOR (São Jose de Tagua, and Playa Grande) (Dyer & Carr, 1990a).Rhinoclemmys melanosterna (Gray, 1861), digestive tract, ECUADOR (Rio Bogotá) (Dyer & Carr, 1990a).Rhinoclemmys nasuta (Boulenger, 1902), digestive tract, ECUADOR (Sarria, Estero el Ceibo, and Playa Grande) (Dyer & Carr, 1990a).Rhinoclemmys punctularia (Daudin, 1801), unspecified site of infection, BRAZIL (Pará State) (Baker & Bain, 1981). Falcaustra sp.Chelonoidis chilensis(Gray, 1870), small intestine, ARGENTINA (San Juan Province) (Castillo et al., 2020).Family Atractidae Travassos, 1919AtractisDujardin, 1845Atractis caballeroiBrenes & Bravo-Hollis, 1960Kinosternon leucostomum Duméril & Bibron, 1851, digestive tract, ECUADOR (Rio Bogotá) (Dyer & Carr, 1990a).Rhinoclemmys annulata (Gray, 1860), digestive tract, ECUADOR (Playa Grande) (Dyer & Carr, 1990a).Atractis cruciata Linstow, 1902Podocnemis expansa (Schweigger, 1812), stomach, BRAZIL (Tocantins State) (Armond, 2008).Atractis dactylurisRudolphi1819Mesoclemmys nasuta(Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Diesing, 1851).Peltocephalus dumerilianus (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Diesing, 1851).Podocnemis expansa (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Diesing, 1851).Atractis impura(Caballero, 1944)Chelonoidis denticulatus(Linnaeus, 1766),intestine, PERU (Loreto and Ucayali Departament) (Salizar & Sanchez, 2007).Rhinoclemmys diademata (Mertens, 1954), digestive tract, VENEZUELA (Lake Maracaib basin) (Dyer & Carr, 1990a). Atractis marqueziBursey & Flanagan 2002Chelonoidis porteri(Rothschild, 1903), colon, ECUADOR (Isla Santa Cruz, Galapagos) (Bursey & Flanagan, 2002).Atractis thapari Petter, 1966Chelonoidis carbonarius (Spix, 1824), small and large intestines, BRAZIL (Piauí State) (Leal et al.,2018; Freire et al., 2019).Chelonoidis denticulatus (Linnaeus, 1766), small and large intestines, BRAZIL (Piauí State) (Leal et al.,2018; Freire et al., 2019).Atractis trematophila Travassos, 1934Amazon river turtle, digestive tract of Nematophila grande(Digenea), BRAZIL (unspecified locality) (Travassos, 1934a).Peltocephalus dumerilianus(Schweigger, 1812), Neotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml103stomach, small and large intestine, BRAZIL () (Ferreira, 2016).Amazonas StateAtractis sp.Podocnemis expansa (Schweigger, 1812), stomach, BRAZIL (Tocantins State) (Armond, 2008).Atractis sp.Chelonoidis carbonarius (Spix, 1824), intestine,VENEZUELA (Bolivar State) (Pérez Mata et al., 2014).Atractis sp. Chelonoidis porteri(Rothschild, 1903), intestine, ECUADOR (Isla Santa Cruz, Galapagos) (Fournié et al., 2015).BuckleyatractisKhalil & Gibbons 1988Buckleyatractis marinkelli Khalil & Gibbons 1988Podocnemis unifilisTroschel, 1848, large intestine, COLOMBIA (unspecified locality) (Khalil & Gibbons, 1988).LabidurisSchneider, 1866Labiduris gulosa(Rudolphi, 1819)Chelonoidis denticulatus(Linnaeus, 1766),intestine, BRAZIL (Pará State) (Chapin, 1924).Mesoclemmys nasuta(Schweigger, 1812), intestine, BRASIL (unspecified locality) (Diesing, 1851).Labiduris irineutaCosta, 1961Chelonoidis denticulatus(Linnaeus, 1766),intestine, BRAZIL (Rio de Janeiro State) (Costa, 1961 cited by Vicente et al., 1993).Labiduris zschokkeiLinstow, 1899Chelonoidis denticulatus(Linnaeus, 1766),intestine, BRAZIL (unspecified locality) (Skrjabin et al.,1964 cited by Vicente et al.,1993).Labiduris sp.Chelonoidis porteri(Rothschild, 1903), intestine, ECUADOR (Isla Santa Cruz, Galapagos) (Fournié et al., 2015).Labiduris sp.Chelonoidis chilensis(Gray, 1870), small intestine, ARGENTINA (San Juan Province) (Castillo et al., 2020).Klossinemella Costa, 1961Klossinemella conciliatus Alho, 1964 Peltocephalus dumerilianus(Schweigger 1812), stomach, small and large intestine, BRAZIL () (Ferreira, 2016).Amazonas StatePodocnemis expansa (Schweigger, 1812), stomach, BRAZIL (Amazonas State) (Alho 1964a;Costa et al.,1968).Podocnemis expansa (Schweigger, 1812), stomach, BRAZIL (Tocantins State) (Armond, 2008).Podocnemis unifilisTroschel, 1848, stomach, PERU (Samiria river) (Salizar & Sanchez, 2007).Klossinemella travassosi Costa, Mota & Gomes, 1968Chelonoidis denticulatus(Linnaeus, 1766), large intestine, BRAZIL (Amazonas State) (Costa et al.,1968; Muniz-Pereira et al.,2009).Klossinemellasp.Peltocephalus dumerilianus(Schweigger, 1812), stomach, small and large intestine, BRAZIL () (Ferreira, 2016).Amazonas StateKlossinemella sp.Peltocephalus dumerilianus(Schweigger, 1812), stomach, small and large intestine, BRAZIL () (Ferreira, 2016).Amazonas StateKlossinemella sp.Peltocephalus dumerilianus(Schweigger, 1812), stomach, small and large intestine, BRAZIL () (Ferreira, 2016).Amazonas StateOrientatractisPetter, 1966Orientatractis leiperi Buckley, 1969Podocnemis vogliMüller, 1935, "stomach" (presumably colon), COLOMBIA (unspecified locality) (Buckley, 1969).Podocnemis unifilisTroschel, 1848, stomach, BRAZIL (Tocantins River, Pará State) (Jesus et al., 2020).Paraorientatractis Gibbons, Khalil & Marinkelle, 1997Paraorientatractis semiannulata Gibbons, Khalil & Marinkelle, 1997Podocnemis unifilisTroschel, 1848, large intestine, BRAZIL (Cuminá and Trombetas rivers near Pará State) (Gibbons et al.,1997).Podocnemis unifilisTroschel, 1848, intestine, Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml104PERU (Ucayali Departament) (Salizar & Sanchez, 2007).ParatractisSarmiento, 1959 Paratractis hystrix (Diesing, 1851)Peltocephalus dumerilianus(Schweigger, 1812), intestine, PERU (Ucayali Departament) Pucallpa, (Sarmiento, 1959).Peltocephalus dumerilianus(Schweigger, 1812), stomach, small and large intestine, BRAZIL () (Ferreira, 2016).Amazonas StatePodocnemis erythrocephala(Spix, 1824), intestine, BRAZIL (Amazonas State) (Diesing, 1851).Podocnemis expansa (Schweigger, 1812), large intestine COLOMBIA (unspecified locality) (Khalil & Gibbons, 1988).Podocnemis unifilisTroschel, 1848, large intestine, COLOMBIA (unspecified locality) (Khalil & Gibbons, 1988).Podocnemis vogliMüller, 1935, unspecified site of infection, COLOMBIA (unspecified locality) (Buckley, 1969; Buckley, 1970). Podocnemis vogliMüller, 1935, large intestine COLOMBIA (unspecified locality) (Khalil & Gibbons, 1988).PneumoatractisBursey, Reavill & Greiner, 2009Pneumoatractis podocnemisBursey, Reavill & Greiner, 2009Podocnemis unifilisTroschel, 1848, lungs, South America (Unknown, wild-caught, confiscated South America turtle) (Bursey et al.,2009).PodocnematractisGibbons, Khalil & Marinkelle, 1995Podocnematractis colombiaensis Gibbons, Khalil & Marinkelle, 1995Podocnemis expansa (Schweigger, 1812), large intestine, COLOMBIA (unspecified locality) (Gibbons et al.,1995).Podocnemis vogliMüller, 1935, large intestine, COLOMBIA (unspecified locality) (Gibbons et al.,1995).Podocnematractisortleppi(Thapar, 1925) Podocnemis expansa (Schweigger, 1812), large intestine, COLOMBIA (unspecified locality) (Gibbonset al.,1995).Podocnemis unifilisTroschel, 1848, large intestine, COLOMBIA (unspecified locality) (Gibbons et al.,1995).Podocnemis vogliMüller, 1935, large intestine, COLOMBIA (unspecified locality) (Gibbons et al.,1995).Superfamily Ascaridoidea Railliet & Henry, 1915Family Anisakidae Skrjabin & Karokhin, 1945 Contracaecum Railliet & Henry, 1912Contracaecum sp. (larvae)Acanthochelys spixii (Duméril & Bibron, 1835), unspecified site of infection, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al.,2017).Family Ascarididae Baird, 1853AngusticaecumBaylis, 1920Angusticaecum brevispiculumChapin, 1924Chelonoidis denticulatus(Linnaeus, 1766), intestine, BRAZIL (Pará State) (Chapin, 1924).Chelonoidis denticulatus(Linnaeus, 1766),intestine, PERU (Loreto and Ucayali Departament) (Salizar & Sanchez, 2007).Brevimulticaecum Mozgovoy, 1951 in Skrjabin, Shikhobalova & Mozgovoi, 1952Brevimulticaecum sp. (larvae)Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (São Paulo State) (Silva, 2014).Superfamily Camallanoidea Travassos, 1920Family Camallanidae Railliet & Henry, 1915Camallanus Railliet & Henry, 1915CamallanusemydidiusMascarenhas & Müller, 2017 Hydromedusa tectiferaCope, 1870, small intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al., 2013; Chaviel et al.,2020). Trachemys dorbigni(Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State), (Mascarenhas & Müller, 2017).Camallanus kachugaeBaylis & Daubney, 1922Kinosternon scorpioides (Linnaeus, 1766), intestine, VENEZUELA (Zulia State) (Díaz-Ungría, 1978).Camallanus sp.Phrynops hilarii (Duméril & Bibron, 1835), stomach and small intestine, BRAZIL (Rio Grande do Sul State) (Bernardon et al.,2013).Camallanus sp.Trachemys dorbigni(Duméril & Bibron, 1835), Neotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml105small intestine, BRAZIL (Rio Grande do Sul State) (Bernardon et al.,2014).Camallanus sp.Acanthochelys spixii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al.,2013).Camallanus sp.Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (Minas Gerais State) (Vieira et al.,2016). SerpinemaYeh, 1960Serpinema amazonicus(Ribeiro, 1940)Podocnemis expansa (Schweigger, 1812), small intestine, BRAZIL (Pará State) (Ribeiro, 1940).Podocnemis expansa (Schweigger, 1812), intestine, PERU (Loreto Departament) (Tantaleán, 1998).Podocnemis unifilisTroschel, 1848, small intestine, PERU (Loreto Departament) (Sánchez et al.,2006).Serpinema cayennensis Harnoster, Svitin & Preez, 2019Rhinoclemmys punctularia(Daudin, 1801), intestine, FRENCH GUIANA (Cayenne) (Harnoster et al.,2019). Serpinema maghati (Sprehn, 1932) Kinosternon integrum Le Conte, 1854, intestine, BOLIVIA (unspecified locality) (Sprehn, 1932).Kinosternon scorpioides (Linnaeus, 1766), small intestine, BRAZIL (Pernambuco, and Pará States) (Alho, 1965; Freitas & Dobbin Jr., 1971).Kinosternon scorpioides (Linnaeus, 1766), stomach, small and large intestine, BRAZIL (Maranhão State) (Viana et al.,2016).Serpinema microcephalus (Dujardin, 1845)Peltocephalus dumerilianus (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Diesing, 1851).Podocnemis expansa (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Diesing, 1851).Serpinema monospiculatus Freitas & Dobbin Jr., 1962 Kinosternon scorpioides (Linnaeus, 1766), large intestine, BRAZIL (Ceará State) (Pereira et al.,2018).Mesoclemmys nasuta (Schweigger, 1812), small intestine, BRAZIL (Pernambuco State) (Freitas & Dobbin Jr., 1971). Mesoclemmys turbeculata(Lüderwaldt, 1926), small intestine, BRAZIL (Pernambuco State) (Freitas & Dobbin Jr., 1971). Mesoclemmys turbeculata(Lüderwaldt, 1926), small and large intestines, BRAZIL (Ceará State) (Pereira et al., 2018). Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (Pernambuco State) (Freitas & Dobbin Jr., 1971). Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (São Paulo State) (Silva, 2014).Phrynops geoffroanus (Schweigger, 1812), stomach, small and large intestines, lungs, body cavity BRAZIL (Ceará State) (Pereira et al.,2018). Spirocamallanus Olsen, 1952Spirocamallanus sp. Hydromedusa tectiferaCope, 1870, small intestine, BRAZIL (Minas Gerais State) (Novelli et al.,2014).Superfamily GnathostomatoideaRailliet, 1895Family GnathostomatidaeRailliet, 1895AncyracanthusDiesing, 1858Ancyracanthus pinnatifidus Diesing, 1839 Peltocephalus dumerilianus (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Diesing, 1851).Peltocephalus dumerilianus(Schweigger, 1812), stomach and intestine, BRAZIL (unspecified locality) (Gomes & Kohn, 1970 cited by Vicente et al.,1993).Peltocephalus dumerilianus(Schweigger, 1812), stomach, BRAZIL () (Ferreira, Amazonas State2016).Podocnemis expansa (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Diesing, 1851).Podocnemis unifilisTroschel, 1848, small intestine, PERU (Loreto Departament) (Sánchez et al.,2006).Gnathostoma Owen, 1836Gnathostoma sp. (larvae)Hydromedusa tectiferaCope, 1870, small intestine, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL 881).SpiroxysSchneider, 1866Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml106Spiroxys contortus(Rudolphi, 1819)Acanthochelys spixii (Duméril & Bibron, 1835), stomach, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al.,2013).Hydromedusa tectiferaCope, 1870, esophagus, stomach, small and large intestines, and cavity, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al.,2013).Phrynops hilarii (Duméril & Bibron, 1835), stomach, ARGENTINA (Buenos Aires Province) (Palumbo et al.,2016).Phrynops hilarii (Duméril & Bibron, 1835), stomach, small and large intestines, BRAZIL (Rio Grande do Sul State) (Chaviel et al.,2020).Trachemys dorbigni(Duméril & Bibron, 1835), esophagus, stomach, small and large intestines, heart, BRAZIL (Rio Grande do Sul State) (Mascarenhas & Müller, 2015b).Spiroxys figueiredoi Freitas & Dobbin Jr., 1962Kinosternon scorpioides (Linnaeus, 1766), stomach, BRAZIL (Pará State) (Alho, 1965).Kinosternon scorpioides (Linnaeus, 1766), stomach, BRAZIL (Pernambuco State) (Freitas & Dobbin Jr., 1962; Freitas & Dobbin Jr., 1971).Kinosternon scorpioides (Linnaeus, 1766), stomach, small and large intestine, BRAZIL (Maranhão State) (Viana et al.,2016).Mesoclemmys turbeculata(Lüderwaldt, 1926), stomach, small intestine, BRAZIL (Ceará State) (Pereira et al.,2018). Phrynops geoffroanus (Schweigger, 1812), stomach, BRAZIL (Ceará State) (Pereira et al.,2018). Phrynops geoffroanus (Schweigger, 1812), stomach, BRAZIL (São Paulo State) (Silva, 2014).Spiroxyssp.Mesoclemmys vanderhaegei (Bour, 1973), stomach, BRAZIL (Mato Grosso State) (Ávila et al.,2010).Spiroxyssp.Phrynops hilarii (Duméril & Bibron, 1835), stomach and small intestine, BRAZIL (Rio Grande do Sul State) (Bernardon et al.,2013).Spiroxyssp.Trachemys dorbigni(Duméril & Bibron, 1835), stomach and small intestine, BRAZIL (Rio Grande do Sul State) (Bernardon et al.,2014).Superfamily Physalopteroidea Sobolev 1949Family Physalopteridae Leiper, 1908 PhysalopteraRudolphi, 1819Physaloptera retusa Rudolphi, 1819Mesoclemmys turbeculata(Lüderwaldt, 1926), large intestine, BRAZIL (Ceará State) (Pereira et al.,2018). Phrynops geoffroanus (Schweigger, 1812), stomach, small intestine, BRAZIL (Ceará State) (Pereira et al.,2018). Physaloptera sp. (larvae)Acanthochelys spixii (Duméril & Bibron, 1835), stomach, BRAZIL (Rio Grande do Sul State) (Chaviel et al.,2020).Physaloptera sp. (larvae)Phrynops geoffroanus (Schweigger, 1812), stomach, BRAZIL (São Paulo State) (Silva, 2014).Superfamily Habronematoidea Railliet & Henry, 1915Family Hedruridae Railliet, 1916HedrurisNitzch, 1821Hedruris dratini Palumbo, Servián, Sánchez & Diaz, 2019Hydromedusa tectiferaCope, 1870, stomach, ARGENTINA (Buenos Aires Province) (Palumbo et al.,2019).Phrynops hilarii (Duméril & Bibron, 1835), stomach, ARGENTINA (Buenos Aires Province) (Palumbo et al.,2019).Hedruris orestiae (Moniez, 1889)Hydromedusa tectiferaCope, 1870, stomach, ARGENTINA (Buenos Aires Province) (Palumbo et al., 2016).Phrynops hilarii (Duméril & Bibron, 1835), stomach, ARGENTINA (Buenos Aires Province) (Palumbo et al., 2016).Hedrurissp.Rhinoclemmys nasuta (Boulenger, 1902), stomach, ECUADOR (Estero el Ceibo) (Dyer & Carr, 1990a).Phylum Platyhelminthes Gegenbaur, 1859Class Trematoda Rudolphi, 1808Subclass Digenea Carus, 1863 Superfamily Diplostomoidea Poirier, 1886Family Proterodiplostomidae Dubois, 1936CheloniodiplostomumSudaricov, 1960Cheloniodiplostomum argentinensisPalumbo & Neotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml107Diaz, 2018Phrynops hilarii (Duméril & Bibron, 1835), anterior portion of intestine, ARGENTINA (Buenos Aires Province)).(Palumbo & Diaz, 2018Cheloniodiplostomum brevis (MacCallum, 1921)Phrynops geoffroanus (Schweigger, 1812), unspecified site of infection, COLOMBIA (unspecified locality) (Dubois, 1979).Cheloniodiplostomum testudinis (Dubois, 1936)Hydromedusa tectiferaCope, 1870, anterior portion of intestine, ARGENTINA (Buenos Aires Province) (Palumbo et al., 2018).Phrynops geoffroanus (Schweigger, 1812), small and large intestines, BRAZIL (São Paulo State) (Silva, 2014).Phrynops hilarii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al., 2016).Phrynops hilarii (Duméril & Bibron, 1835), intestine, ARGENTINA (Corrientes Province) (Lombardero & Moriena, 1977).Phrynops hilarii (Duméril & Bibron, 1835), anterior portion of intestine, ARGENTINA (Buenos Aires Province) (Palumbo et al., 2018).Testudo sp., unspecified site of infection, BRAZIL (unspecified locality) (Dubois 1936 cited byFernandes & Kohn, 2014).Cheloniodiplostomum sp.Phrynops hilarii (Duméril & Bibron, 1835), stomach and small intestine, BRAZIL (Rio Grande do Sul State) (Bernardon et al., 2013).Cheloniodiplostomum sp.Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (Minas Gerais State) (Novelli et al.,2013).Cheloniodiplostomum sp.Acanthochelys spixii (Duméril & Bibron, 1835), small and large intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al., 2016).Cheloniodiplostomum sp.Trachemys dorbigni(Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Bernardon et al., 2014).Cheloniodiplostomum sp.Hydromedusa tectiferaCope, 1870, small intestine, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL 885).Superfamily Schistosomatoidea Stiles & Hassall, 1898Family Spirorchiidae Stunkard, 1921AtamatamBullard & Roberts, 2019Atamatam amazoniensisBullard & Roberts, 2019Chelus fimbriata(Schneider, 1783), blood vessels of kidney and mesentery, PERU (Iquitos Departament) (Bullard et al., 2019).ParatamatamBullard & Roberts, 2019Paratamatam iquitosiensis Bullard & Roberts, 2019Chelus fimbriata (Schneider, 1783), blood vessels of kidney and mesentery, PERU (Iquitos Departament) (Bullard et al., 2019).Superfamily Echinostomatoidea Looss, 1899Family Echinostomatidae Looss, 1899PrionosomoidesFreitas & Dobbin Jr., 1967Prionosomoidesphrynopsis (Mañé-Garzón & Gil, 1961) Phrynops hilarii (Duméril & Bibron, 1835), small intestine, URUGUAY (Tacuarembó Departament) (Mañé-Garzón & Gil, 1961a).Prionosomoides scalarisFreitas & Dobbin Jr., 1967Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (Pernambuco State) (Freitas & Dobbin Jr., 1967).Phrynops hilarii (Duméril & Bibron, 1835), esophagus, ARGENTINA (Corrientes Province) (Lombardero & Moriena, 1977).Prionosomoidessp. Phrynops hilarii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL, 886-887).Family Rhytidodidae Odhner, 1926Rhytidodes Looss, 1901Rhytidodes gelatinosus (Rudolphi, 1819)Podocnemis expansa (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Travassos et al.,1969). Superfamily Heronimoidea Ward, 1917Family Heronimidae Ward, 1917Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml108Heronimus MacCallum, 1902Heronimus mollis (Leidy, 1856)Trachemys callirostris(Gray, 1856), lungs, COLOMBIA (unspecified locality) (Lenis & Vélez, 2011).Superfamily Microscaphidioidea Looss, 1900Family Microscaphidiidae Looss, 1900NeodeuterobarisBrooks, 1976Neodeuterobaris pritchardae Brooks, 1976 Podocnemis lewyanaDuméril, 1852, stomach, COLOMBIA (Caldas Departament) (Brooks, 1976).Podocnemis lewyanaDuméril, 1852, stomach, COLOMBIA (Magdalena river) (Lenis & Vélez, 2011).OctangioidesPrice, 1937Octangioides tlacotalpensisCaballero, 1942Rhinoclemmys nasuta (Boulenger, 1902), large intestine, ECUADOR (Esmeraldas Province) (Dyer & Carr, 1990b).Podocnemitrema Alho & Vicente, 1964Podocnemitrema papillosusAlho & Vicente, 1964Podocnemis expansa (Schweigger, 1812), stomach, BRAZIL (Amazonas State) (Alho & Vicente, 1964).Podocnemis expansa (Schweigger, 1812), digestive tract, PERU (Iquitos Departament) (Tantaleán et al.,2011).Podocnemis unifilisTroschel, 1848, digestive tract, PERU (Iquitos Departament) (Tantaleán et al.,2011).Superfamily Paramphistomatoidea Fischoeder, 1901Family Cladorchiidae Fischoeder, 1901HalltremaLent & Freitas, 1939Halltrema avitellina Lent & Freitas, 1939Chelonoidis denticulatus (Linnaeus, 1766), stomach, BRAZIL (Pará State) (Alho, 1965).Podocnemis expansa (Schweigger, 1812), stomach, BRAZIL (Tocantins State) (Armond, 2008).Podocnemis expansa (Schweigger, 1812), unspecified site of infection, BRAZIL (Pará State) (Lent & Freitas, 1939; Freitas & Lent, 1942 cited by Fernandes & Kohn, 2014).Podocnemis expansa (Schweigger, 1812), digestive tract, PERU (Iquitos Departament) (Tantaleán et al., 2011).Podocnemis unifilisTroschel, 1848, digestive tract, PERU (Iquitos Departament) (Tantaleán et al., 2011).Podocnemis sp., unspecified site of infection, VENEZUELA (unspecified locality) (Caballero & Diaz-Ungria, 1958 cited by Fernandes & Kohn, 2014).Halltrema heteroxenus (Cordero & Vogelsang, 1940)Podocnemis unifilisTroschel, 1848, digestive tract, PERU (Loreto Departament) (Tantaleán & Forlong, 2013).Podocnemis sp., stomach, VENEZUELA (Guarico State) (Cordero & Vogelsang, 1940).Rhinoclemmys nasuta(Boulenger, 1902), digestive tract, ECUADOR (Esmeraldas Province) (Dyer & Carr, 1990b).Nematophila Travassos, 1934Nematophila argentinum (Cordero & Vogelsang, 1940) Phrynops sp., intestine, ARGENTINA (Misiones Province) (Cordero & Vogelsang, 1940).Podocnemis lewyanaDuméril, 1852, large intestine, COLOMBIA (Bolívar Departament) (Lenis & Vélez, 2011).Trachemys callirostris(Gray, 1856), large intestine, COLOMBIA (Bolívar Departament) (Lenis & Vélez, 2011).Nematophila grandis (Diesing, 1839)Chelus fimbriata(Schneider, 1783), intestine, BRAZIL (unspecified locality) (Travassos, 1934b; Travassos et al.,1969).Hydraspis schopfii, intestine, BRAZIL (unspecified locality) (Travassos, 1934b).Hydromedusa tectiferaCope, 1870, unspecified site of infection, PARAGUAY (unspecified locality) (Masi-Pallarés et al., 1976 cited by Fernandes & Kohn, 2014).Kinixys erosa (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Travassos et al., 1969; Fernandes & Kohn, 2014).Kinosternon scorpioides (Linnaeus, 1766), small and large intestine, BRAZIL (Pará State) (Alho, 1964b).Kinosternon scorpioides (Linnaeus, 1766), stomach, VENEZUELA (Zulia State) (Díaz-Ungría, 1978).Mesoclemmys gibba (Schweigger, 1812), intestine, Neotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml1092014).Rhinoclemmys nasuta(Boulenger, 1902), small and large intestines, ECUADOR (Esmeraldas Province) (Dyer & Carr, 1990b).Rhinoclemmys punctularia(Daudin, 1801),large intestine, BRAZIL (Pará State) (Alho, 1964b).Rhinoclemmys punctularia (Daudin, 1801), digestive tract, GUYANA FRANCESA (Iracoubo) (Dyer & Carr, 1990b).Nematophila venezuelenisis (Cordero & Vogelsang, 1940)Podocnemis lewyanaDuméril, 1852, stomach, COLOMBIA (Bolívar and Magdalena Departaments) (Lenis & Vélez, 2011).Podocnemis sp., stomach, VENEZUELA (Guarico, and Bolívar State) (Cordero & Vogelsang ,1940). OriximinatremaKnoff, Brooks, Mullins & Gomes, 2012Oriximinatrema noronhae Knoff, Brooks, Mullins & Gomes, 2012Podocnemis expansa (Schweigger, 1812), stomach and intestine, BRAZIL (Pará State) (Knoff et al., 2012).Pseudocleptodiscus Caballero, 1961Pseudocleptodiscus margaritae Caballero, 1961Rhinoclemmys nasuta(Boulenger, 1902), small and large intestines, ECUADOR (Esmeraldas Province) (Dyer & Carr, 1990b).Pseudonematophila Lenis & Vélez, 2011Pseudonematophila ovalis (Cordero & Vogelsang, 1940) Podocnemis sp., stomach, VENEZUELA (Guárico State) (Cordero & Vogelsang, 1940).Podocnemis lewyanaDuméril, 1852, stomach, COLOMBIA (Bolívar and Magdalena Departaments) (Lenis & Vélez, 2011).Superfamily Allocreadioidea Looss, 1902Family Opecoleidae Ozaki, 1925Helicotrema Odhner, 1902Helicotrema spirale (Diesing, 1850)Chelonoidis denticulatus(Linnaeus, 1766), small intestine, PERU (Iquitos Departament) (Tantaleán & Forlong, 2013).Chelonoidis denticulatus(Linnaeus, 1766), intestine, BRAZIL (unspecified locality) (Odhner, 1912 cited by Fernandes & Kohn, 2014).BRAZIL (unspecified locality) (Travassos, 1934b; Travassos et al.,1969).Mesoclemmys nasuta (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Travassos, 1934b; Travassos et al.,1969).Peltocephalus dumerilianus(Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Travassos, 1934b; Travassos et al.,1969).Peltocephalus dumerilianus(Schweigger, 1812), stomach, small and large intestine, BRAZIL () (Ferreira, 2016).Amazonas StatePhrynops geoffroanus (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Travassos, 1934b; Travassos et al.,1969).Phrynops geoffroanus (Schweigger, 1812), large intestine, BRAZIL (São Paulo State) (Silva, 2014).Phrynops hilarii (Duméril & Bibron, 1835), intestine, ARGENTINA (Corrientes Province) (Lombardero & Moriena, 1977).Podocnemis expansa (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Travassos et al., 1969).Podocnemis expansa (Schweigger, 1812), stomach, PERU (Loreto Departament) (Tantaleán et al.,2011).Podocnemis expansa (Schweigger, 1812), stomach, VENEZUELA (Zulia State) (Díaz-Ungría, 1978).Podocnemis erythrocephala(Spix, 1824), unspecified site of infection, BRAZIL (unspecified locality) (Diesing, 1850 cited by Fernandes & Kohn, 2014).Podocnemis unifilisTroschel, 1848, stomach, and intestine, PERU (Loreto, and Madre de Dios Departaments) (Salizar & Sanchez, 2004).Podocnemis unifilisTroschel, 1848, stomach, and large intestine, PERU (Loreto Departament) (Sánchez et al., 2006).Podocnemis unifilisTroschel, 1848, stomach, PERU (Loreto Departament) (Tantaleán et al., 2011).Podocnemis unifilisTroschel, 1848, unspecified site of infection, VENEZUELA (unspecified locality) (Heyneman et al., 1960 cited by Fernandes & Kohn, 2014).Podocnemis vogli Müller, 1935, unspecified site of infection, VENEZUELA (unspecified locality) (Heyneman et al., 1960 cited by Fernandes & Kohn, 2014).Podocnemis sp., unspecified site of infection, VENEZUELA (unspecified locality) (Caballero & Diaz-Ungria, 1958 cited by Fernandes & Kohn, Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml110Podocnemis expansa (Schweigger, 1812), small intestine, BRAZIL (Pará State) (Lent & Freitas, 1938).Superfamily Plagiorchioidea Lühe, 1901Family Telorchiidae Looss, 1899Loefgrenia Travassos, 1920Loefgrenia loefgreniTravassos, 1919Podocnemis unifilisTroschel, 1848, intestine, BRAZIL (unspecified locality) (Travassos, 1919 cited by Fernandes & Kohn, 2014).Pseudotelorchis Yamaguti, 1971Pseudotelorchis devincenzii (Mañé-Garzón & Gil, 1961)Hydromedusa tectiferaCope, 1870, small intestine, URUGUAY (Canelones Departament) (Mañé-Garzón & Gil, 1961b).TelorchisLühe, 1899Telorchis achavaliMañé-Garzón & Holcman-Spector, 1973Trachemys dorbigni(Duméril & Bibron, 1835), small intestine, URUGUAY (Tacuarembó Departament) (Mañé-Garzón & Holcman-Spector, 1973).Trachemys dorbigni(Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas & Müller, 2013).Telorchis aculeatus (Linstow, 1879)Podocnemis unifilisTroschel, 1848, intestine, VENEZUELA (Sucre State) (Nasir, 1974).Telorchis bifurcus (Braun, 1899)Podocnemis expansa (Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Braun, 1901 cited by Fernandes & Kohn, 2014).Telorchis birabeniMañé-Garzón & Gil, 1961Phrynops hilarii (Duméril & Bibron, 1835), small intestine, URUGUAY (Tacuarembó Departament) (Mañé-Garzón & Gil, 1961c).Phrynops hilarii (Duméril & Bibron, 1835), small intestine, ARGENTINA (Corrientes Province) (Lombardero & Moriena, 1977).Phrynops hilarii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al., 2016).Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (São Paulo State) (Silva, 2014).Telorchis cortiStunkard, 1915 Peltocephalus dumerilianus(Schweigger, 1812), intestine, BRAZIL (unspecified locality) (Odhner, 1912 cited by Fernandes & Kohn 2014).Peltocephalus dumerilianus(Schweigger, 1812), small intestine, BRAZIL () Amazonas State(Ferreira, 2016).Superfamily Opisthorchioidea Looss, 1899Family Cryptogonimidae Ward,1917Acanthostomum Looss, 1899 Acanthostomum scyphocephalus(Braun, 1899)Chelus fimbriata(Schneider, 1783), intestine, BRAZIL (unspecified locality) (Ostrowski de Núñez, 1986).Caimanicola Freitas & Lent, 1938 Caimanicolabrauni (Mañé-Garzón & Gil, 1961)Acanthochelys spixii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Chaviel et al.,2020).Phrynops hilarii (Duméril & Bibron, 1835), small intestine, URUGUAY (Tacuarembó Departament) (Mañé-Garzón & Gil, 1961a).Phrynops hilarii (Duméril & Bibron, 1835), ARGENTINA (Ostrowski de Núñez 1987 [experimental study] cited by Fernandes & Kohn, 2014).Phrynops hilarii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Chaviel et al.,2020).TimoniellaRebecq, 1960Timoniella ostrowski Brooks & Holcman, 1993Phrynops hilarii (Duméril & Bibron, 1835), small intestine, URUGUAY (Tacuarembó Departament) (Mañé-Garzón & Gil, 1961a; Brooks & Holcman, 1993).Phrynops hilarii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Chaviel et al.,2020).Superfamily Gorgoderoidea Looss, 1899Family Gorgoderidae Looss, 1899Gorgoderina Looss, 1902Gorgoderina sp.Phrynops geoffroanus (Schweigger, 1812), small intestine, BRAZIL (Ceará State) (Pereira et al.,2018). Family Braunotrematidae Yamaguti, 1958Braunotrema Price, 1930Braunotrema pulvinatum(Braun, 1899)Neotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml111intestine, URUGUAY (Tacuarembó Departament) (Mañé-Garzón & Gil, 1961b).Telorchis rapidulus Dobbin Jr., 1957Kinosternon scorpioides (Linnaeus, 1766), small intestine, BRAZIL (Pernambuco State) (Dobbin Jr., 1957).Telorchis sp.Hydromedusa tectiferaCope, 1870, small intestine, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL 893-896).Telorchis sp.Trachemys dorbigni(Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas, 2014).Class Cestoda Van Beneden, 1849Order Proteocephalidea Mola, 1928Family Proteocephalidae La Rue, 1911Ophiotaenia La Rue, 1911Ophiotaenia cohospesCordero, 1946 Hydromedusa tectiferaCope, 1870, intestine, URUGUAY (Montevideo) (Cordero 1946).Ophiotaenia lopesiRego, 1967Chelonoidis denticulatus(Linnaeus, 1766),smallintestine, BRAZIL (Pará State) (Schmidt, 1986; Muniz-Pereira et al.,2009).Ophiotaenia sp. Hydromedusa tectiferaCope, 1870, smallintestine, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL, 890-892).Class Monogenoidea Bychowsky, 1937Order Polystomatidea Lebedev, 1988Family Polystomatidae Gamble, 1896Polystomoides Ward, 1917Polystomoides brasiliensis Vieira, Noveli, Sousa & Souza-Lima, 2008Hydromedusa maximiliani (Mikan, 1820), buccal and pharyngeal cavities, BRAZIL (Minas Gerais State) (Vieira et al.,2008).Mesoclemmys turbeculata(Lüderwaldt, 1926), buccal and pharyngeal cavities, BRAZIL (Sergipe State) (Santana et al.,2019).Phrynops geoffroanus (Schweigger, 1812), buccal and pharyngeal cavities, BRAZIL (Minas Gerais State) (Vieira et al.,2008).Phrynops geoffroanus (Schweigger, 1812), mouth Trachemys callirostris (Gray, 1856), small intestine, COLOMBIA (unspecified locality) (Lenis & Vélez, 2011).Trachemys dorbigni(Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas & Müller, 2013).Telorchis diaphanusFreitas & Dobbin Jr., 1959Kinosternon scorpioides (Linnaeus, 1766), small intestine, BRAZIL (Pernambuco State) (Freitas & Dobbin Jr., 1959).Telorchis dubiusMañé-Garzón & Holcman-Spector, 1968Trachemys dorbigni(Duméril & Bibron, 1835), small intestine, URUGUAY (Flores Departament) (Mañé-Garzón & Holcman-Spector, 1968a).Telorchis hagmanni Lent & Freitas, 1937Peltocephalus dumerilianus(Schweigger, 1812), small intestine, BRAZIL () Amazonas State(Ferreira, 2016).Podocnemis expansa (Schweigger, 1812), stomach, and small intestine, BRAZIL (Pará State) (Lent & Freitas, 1937; Alho, 1965).Podocnemis lewyanaDuméril, 1852, small intestine, COLOMBIA (Bolívar and Magdalena Departaments) (Lenis & Vélez, 2011).Podocnemis unifilisTroschel, 1848, digestive tract, PERU (Iquitos Departament) (Tantaleán et al., 2011).Telorchis parvus Braun, 1901Emys orbicularis (Linnaeus, 1758), intestine, BRAZIL (unspecified locality) (Braun, 1901 cited by Fernandes & Kohn, 2014).Telorchis platensis Mañé-Garzón & Gil, 1961Acanthochelys spixii (Duméril & Bibron, 1835), large intestine, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al., 2016).Hydromedusa tectiferaCope, 1870, small intestine, URUGUAY (Canelones Departament) (Mañé-Garzón & Gil, 1961b).Telorchis pleroticus (Braun, 1899)Freshwater turtle, intestine, BRAZIL (unspecified locality) (Braun, 1901 cited by Fernandes & Kohn, 2014).Telorchis productusMañé-Garzón & Gil, 1961Phrynops hilarii (Duméril & Bibron, 1835), small Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml112State) (Mascarenhas, 2014).Neopolystomasp.Mesoclemmys vanderhaegei (Bour, 1973), unspecified site of infection, BRAZIL (Mato Grosso State) (Ávila et al., 2010).Superfamily Iagotrematoidea Mañé-Garzón & Gil, 1962Family Iagotrematidae Mañé-Garzón & Gil, 1962Iagotrema Mañé-Garzón & Gil, 1962Iagotrema uruguayensisMañé-Garzón & Gil, 1962Hydromedusa tectiferaCope, 1870, urinary bladder, URUGUAY (Tacuarembó Department) (Mañé-Garzón & Gil, 1962b). Infraclass Rhabdocoela Ehrenberg, 1831 Order Dalytyphloplanida Williems et al., 2006Infraorder Temnocephalida Blanchard, 1849Superfamily Temnocephaloidea Baer, 1953Family Temnocephalidae Monticelli, 1899Temnocephala Blanchard, 1849Temnocephala brevicornisMonticelli 1889Acanthochelys radiolata (Mikan, 1820), BRAZIL (unspecified locality) (Monticelli 1889 cited by Martínez-Aquinoet al., 2014).Acanthochelys spixii (Duméril & Bibron, 1835), skin of the neck, axillary, inguinal region, BRAZIL (Rio Grande do Sul State) (Yuki et al., 1993).Hydromedusa tectiferaCope, 1870, plastron and the skin of the axillary, inguinal and anal regions, BRAZIL (São Paulo State) (Pereira & Cuocolo, 1940).Hydromedusa tectiferaCope, 1870, URUGUAY (Tacuarembó Departament) (Dioni, 1967 cited by Martínez-Aquinoet al., 2014).Hydromedusa tectiferaCope, 1870, URUGUAY (Montevideo) (Cordero, 1946).Hydromedusa tectiferaCope, 1870, surface, ARGENTINA (Buenos Aires Province) (Brusa & Damborenea, 2000; Martínez-Aquinoet al.,2014).Hydromedusa maximiliani (Mikan, 1820), plastron and the skin of the axillary, inguinal and anal regions, BRAZIL (Rio de Janeiro) (Pereira & Cuocolo, 1940).Hydromedusa maximiliani (Mikan, 1820), plastron and the skin of the axillary, inguinal and anal regions, BRAZIL (Minas Gerais State) (Pereira & Cuocolo, 1940).Hydromedusa maximiliani (Mikan, 1820), and esophagus, BRAZIL (São Paulo State) (Silva, 2014).Phrynops geoffroanus (Schweigger, 1812), buccal and pharyngeal cavities, BRAZIL (Sergipe State) (Santana et al., 2019).Polystomoides fuquesiMañé-Garzón & Gil, 1962Phrynops hilarii (Duméril & Bibron, 1835), oral cavity, URUGUAY (Artigas Departament) (Mañé-Garzón & Gil, 1962a).Polystomoides magdalenensis Lenis & García-Prieto, 2009Trachemys callirostris(Gray, 1856), oral cavity, COLOMBIA (Bolivar Departament) (Lenis & García-Prieto, 2009).Polystomoides rohdeiMañé-Garzón & Holcman-Spector, 1968 Trachemys dorbigni(Duméril & Bibron, 1835), oral cavity, URUGUAY (Florida, Soriano, and Rivera Departaments) (Mañé-Garzón & Holcman-Spector, 1968b).Trachemys dorbigni(Duméril & Bibron, 1835), oral cavity, BRAZIL (Rio Grande do Sul State), (Mascarenhas, 2014).Polystomoides uruguayensis Mañé-Garzón & Gil, 1961Phrynops hilarii (Duméril & Bibron, 1835), oral cavity, URUGUAY (Artigas Departament) (Mañé-Garzón & Gil, 1961d).Polystomoidessp.Acanthochelys spixii (Duméril & Bibron, 1835), oral cavity, BRAZIL (Rio Grande do Sul State) (Chaviel et al., 2020).Polystomoidessp.Phrynops geoffroanus (Schweigger, 1812), urinary bladder, BRAZIL (São Paulo State) (Silva, 2014).Polystomoidessp.Phrynops hilarii (Duméril & Bibron, 1835), oral cavity, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL 902).Neopolystoma Price, 1939Neopolystomasp.Trachemys dorbigni(Duméril & Bibron, 1835), urinary bladder, BRAZIL (Rio Grande do Sul Neotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml113of the carapace and epidermis of the bases of neck and limbs, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL 897-901).Phylum Acanthocephala Rudolphi, 1808Class Palaeacanthocephala Meyer, 1931Order Polymorphida Petrochenko, 1956Family Polymorphidae Meyer, 1931Polymorphidae gen. sp. (immature)Acanthochelys spixii (Duméril & Bibron, 1835), small intestine, BRAZIL (Rio Grande do Sul State) (present study, CHLAPASIL 888).HOST-HELMINTH LIST CryptodiraKinosternoideaKinosternidaeKinosternon integrum (IUCN:Least concern)Serpinema maghati Kinosternon leucostomum Atractis caballeroiKinosternon scorpioidesCamallanus kachugae Nematophila grandisSerpinema maghati Serpinema monospiculatus Spiroxys figueiredoi Telorchis diaphanusTelorchis rapidulusTestudinoideaEmydidaeEmys orbicularis (IUCN: near threatened)Telorchis parvusTrachemys callirostrisHeronimus mollis Nematophila argentinumPolystomoides magdalenensisTelorchis cortiTrachemys dorbigni CamallanusemydidiusCamallanus sp.Cheloniodiplostomum sp.Dioctophyme renale(larvae)Eustrongylidessp. (larvae)Falcaustra affinisNeopolystomasp.Polystomoides rohdeiepidermis of the neck, axillary, inguinal and anal region and plastron, BRAZIL (Minas Gerais State) (Novelli et al., 2009).Mesoclemmys gibba (Schweigger, 1812), BRAZIL (Monticelli, 1899 cited by Pereira & Cuocolo, 1940).Phrynops hilarii (Duméril & Bibron, 1835), ARGENTINA (Buenos Aires Province) (Martínez-Aquinoet al., 2014).Trachemys dorbigni(Duméril & Bibron, 1835), skin of the neck, axillary, inguinal and anal areas, and plastron, BRAZIL (Rio Grande do Sul State) (Yuki et al., 1993).Temnocephala cuocoloi Volonterio, 2010Hydromedusa tectiferaCope, 1870, surface of the plastron, URUGUAY (Canelones Departament) (Volonterio, 2010).Temnocephala pereirai Volonterio, 2010Hydromedusa tectiferaCope, 1870, surface of the plastron, URUGUAY (Canelones Departament) (Volonterio, 2010).Trachemys dorbigni(Duméril & Bibron, 1835), skin of the neck, axillary, inguinal and perianal areas, and in the surface of plastron, BRAZIL (Rio Grande do Sul State) (Seixas et al., 2014; Mascarenhas et al., 2018).Temnocephala sp.Trachemys dorbigni(Duméril & Bibron, 1835), skin of the neck, axillary, inguinal and perianal areas, and plastron, BRAZIL (Rio Grande do Sul State) (Mascarenhas et al., 2018).Temnocephala sp.Hydromedusa tectiferaCope, 1870, body, limbs and hull,BRAZIL (Rio Grande do Sul State) (Soares et al., 2007).Temnocephala sp.Hydromedusa tectiferaCope, 1870, dorsal surface of the plastron, ventral surface of the carapace and bridges, epidermis adjacent to the ventral surface of the carapace and epidermis of the bases of neck and limbs,BRAZIL (Rio Grande do Sul State) (Huckembeck & Quintela, 2013).Temnocephala sp.Hydromedusa tectiferaCope, 1870, surface of the plastron, ventral surface of the carapace and bridges, epidermis adjacent to the ventral surface Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml114Klossinemella travassosiLabiduris gulosaLabiduris irineutaLabiduris zschokkeiOphiotaenia lopesiSauricola sauricolaChelonoidis porteri(IUCN: Critically endangered)Atractis marqueziAtractissp.Labidurissp.Kinixys erosa (IUCN: Data deficient)Nematophila grandisTestudo sp.Cheloniodiplostomum testudinisPleurodira Chelidae Acanthochelys radiolata (IUCN: Near threatened)Temnocephala brevicornisAcanthochelys spixii (IUCN: Near threatened)CaimanicolabrauniCamallanussp.Cheloniodiplostomumsp.Contracaecum sp. (larvae)Dioctophyme renale (larvae)Pharyngodonidae gen. sp.Physalopterasp. (larvae)Polymorphidae gen. sp. (immature)Polystomoidessp.Spiroxys contortus TelorchisplatensisTemnocephala brevicornisChelus fimbriataAcanthostomum scyphocephalusAtamatam amazoniensisNematophila grandisParatamatam iquitosiensisMesoclemmys gibbaNematophila grandisTemnocephala brevicornisMesoclemmys nasutaAtractis dactylurisLabiduris gulosaNematophila grandisSpiroxyscontortusSpiroxyssp.Telorchis achavaliTelorchis cortiTelorchis dubiusTelorchissp.Temnocephala brevicornisTemnocephala pereiraiTemnocephala sp.GeoemydidaeRhinoclemmys annulata(IUCN: near threatened)Atractis caballeroiFalcaustra tikasinghiRhinoclemmys diademataAtractis impureRhinoclemmys melanosternaFalcaustra tikasinghiRhinoclemmys nasuta (IUCN: near threatened)Falcaustra tikasinghiHalltremaheteroxenusHedruris sp.Nematophila grandis Octangioides tlacotalpensis Pseudocleptodiscus margaritaeRhinoclemmys punctulariaFalcaustra tikasinghiNematophila grandisSerpinema cayennensisTestudinidaeChelonoidis carbonarius Atractis thapariAtractissp.Chapiniella variabilisThelandros sp.Chelonoidis chilensis (IUCN: Vulnerable)Falcaustra sp.Labidurissp.Chelonoidis denticulatus(IUCN: Vulnerable)Angusticaecum brevispiculumAtractis impureAtractis thapariChapiniella variabilisHalltrema avitellinaHelicotrema spiraleNeotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml115Phrynopssp.Nematophila argentinumHydromedusa maximiliani(IUCN: Vulnerable) Polystomoides brasiliensis Temnocephala brevicornis Hydromedusa tectiferaCamallanusemydidiusCheloniodiplostomum testudinisCheloniodiplostomumsp.Dioctophyme renale(larvae)Gnathostoma sp. (larvae)Hedruris dratiniHedruris orestiaeIagotrema uruguayensisNematophila grandisOphiotaenia cohospesOphiotaenia sp. Pseudotelorchis devincenziiSpirocamallanussp.Spiroxys contortusTelorchis platensis Telorchis sp.Temnocephala brevicornisTemnocephala cuocoloi Temnocephala pereiraiTemnocephalasp. Hydraspis schopfiiNematophila grandis PodocnemididaePeltocephalus dumerilianus(IUCN: Vulnerable)Ancyracanthus pinnatifidus Atractis dactylurisAtractis trematophilaHelicotrema spirale Klossinemella conciliatusKlossinemella sp.Nematophila grandisParatractis hystrixSerpinema microcephalusTelorchis hagmanniPodocnemis erythrocephala(IUCN: Vulnerable)Nematophila grandisParatractis hystrix Podocnemis expansa (IUCN: Lower Risk/conservation dependen)Ancyracanthus pinnatifidus Serpinema monospiculatusMesoclemmys turbeculataPhysaloptera retusaPolystomoides brasiliensisSerpinema monospiculatusSpiroxys figueiredoiMesoclemmys vanderhaegei (IUCN: Near threatened)Neopolystomasp.Spiroxyssp.Phrynops geoffroanusBrevimulticaecumsp. (larvae)Camallanussp.Cheloniodiplostomum brevisCheloniodiplostomum testudinisCheloniodiplostomumsp.Gorgoderina sp.Nematophila grandisPhysalopterasp. (larvae)Physaloptera retusaPolystomoides brasiliensisPolystomoides sp.Prionosomoides scalarisSerpinema monospiculatusSpiroxys figueiredoiTelorchis birabeniPhrynops hilariiCaimanicola brauniCamallanussp.Cheloniodiplostomum argentinensisCheloniodiplostomum testudinisCheloniodiplostomumsp. Dioctophyme renale(larvae)Hedruris dratiniHedruris orestiaeNematophila grandisPolystomoides fuquesiPolystomoides uruguayensisPolystomoidessp.Prionosomoides phrynopsisPrionosomoides scalarisPrionosomoides sp.SpiroxyscontortusSpiroxyssp. Telorchis birabeniTelorchis productusTemnocephala brevicornisTimoniella ostrowoskiNeotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
image/svg+xml116Podocnemissp.Halltrema avitellinaHalltremaheteroxenus Nematophila grandisNematophila venezuelenisis Pseudonematophila ovalis Freshwater turtle Telorchis pleroticusAmazon river turtleAtractis trematophilaAtractis cruciateAtractis dactylurisAtractis sp.Braunotrema pulvinatumHalltrema avitellina Klossinemella conciliatus Nematophila grandisOriximinatrema noronhaeParatractis hystrix Podocnematractis colombiaensis PodocnematractisortleppiPodocnemitrema papillosusRhytidodes gelatinosus Serpinema amazonicus Serpinema microcephalusTelorchis bifurcusTelorchis hagmanniPodocnemis lewyana (IUCN: Critically Endangered)Nematophila argentinumNematophila venezuelenisis Neodeuterobaris pritchardae Pseudonematophila ovalisTelorchis hagmanni Podocnemis unifilis(IUCN: Vulnerable)Ancyracanthus pinnatifidus Buckleyatractis marinkelliHalltrema avitellinaHalltrema heteroxenusKlossinemella conciliatus Loefgrenia loefgreniaNematophila grandisNematophilasp.Orientatractis leiperiParaorientatractis semiannulata Paratractis hystrix Pneumoatractis podocnemisPodocnematractisortleppiPodocnemitrema papillosusSerpinema amazonicusTelorchis aculeatusTelorchis hagmanniPodocnemis vogliNematophila grandisOrientatractis leiperiParatractis hystrix Podocnematractis colombiaensis PodocnematractisortleppiCohenet al.(2013) carried out a list of Monogenoidea associated with Testudines from South America in which they cited eight records. This checklist added nine records of Polystomatidae. Fernandes & Kohn (2014) introduced a checklist of Trematoda, in which they cited 34 taxaregistered in the region. This checklist added 15 taxato this group of parasites. Martínez-Aquino et al. (2014) gathered the records of Temnocephalida in the Neotropical region and cited four taxaassociated with freshwater turtles from South America. This checklist added three taxaassociated with these vertebrates and included the record of Temnocephala pereiraiin Trachemys dorbigniin Brazil (Seixas et al., 2014; Mascarenhas et al., 2018). In addition, this checklist has gathered the records of Nematoda, Cestoda and Acanthocephala parasites of Testudines from South America. Thus, it should be highlighted that these vertebrates host a little-known rich fauna of helminths, that many Testudines have been poorly studied and that several studies have only been carried out with the examination of their digestive tract (e.g. Sanchez et al., 2006; Tantaleán et al., 2011; Tantaleán & Forlong, 2013; Viana et al., 2016). Besides the stomach and intestines (main reported sites of infection), parasitic helminths can be found in blood vessels (Bullard et al., 2019), lungs (Bursey et al.,2009; Lenis & Vélez, 2011), the urinary bladder (Mañé-Garzón & Gil, 1962; Mascarenhas, 2014) and in other sites of infection that have not been reported yet.Concerning the conservation status of continental Testudines from South America, many species are DISCUSSIONNeotropical Helminthology, 2021, 15(1), ene-junMascarenhas & Müller
image/svg+xml117inventories of wildlife associated helminths, particularly in biomes and host species that have not been sampled because the missing information is even greater than the current state of knowledge, as observed in the present review. In this context, helminthological studies of Testudines generate information that can be used in studies that aim at the conservation of the host species and, consequently, of their associated organisms, as well as the sites that sustain these invaluable relations. The authors would like to thank Marco Antonio Afonso Coimbra for his assistance. This study was supported by CAPES (Coordenação de Aperfeiçoamento do Pessoal de Nível Superior, process number 32/2010).threatened. According to Rhodin et al.(2017), Testudines are one of the most threatened major groups of vertebrates, in general more than birds, mammals, cartilaginous or bony fishes and amphibians. In this scenario, several helminths associated with these hosts can either be potentially impacted or even extinct without at least being described or recorded. Less than half of native South American species cited in the IUCN list have had helminthological records. Peltocephalus dumerilianus(VU), Podocnemis unifilis (VU) and Chelonoides denticulatus(VU) are the most studied ones, while Chelonoides chilensis (VU), Podocnemis erythrocephala (VU), Podocnemis lewyana(CR) and Chelonoides porteri(CR) are the least studied ones; two records of helminths in thP. erythrocephaladate from the 19 century (Diesing, 1850 cited by Fernandes & Kohn, 2014; Diesing, 1851). Studies of P. lewyana, C. chilensis, and C. porteriare more recent (Brooks, 1976; Bursey & Flanagan, 2002; Lenis & Vélez, 2011; Fournié et al., 2015; Castillo et al., 2020). As for ectosymbionts, it should be highlighted that Temnocephalacomprises freshwater species endemic to the Neotropical region that have specificity regarding the host group and/or species (Martínez-Aquinoet al., 2014). Only seven out of 71 continental Testudines taxafrom South America were reported as hosts of Temnocephala; three species were listed as near threatened (NT) by the IUCN. Therefore, conservation actions related to Testudines can contribute to the conservation of several other organisms that depend on these vertebrates. Parasites are regulators of host populations and powerful agents that maintain the stability of ecosystems. Many species of helminths, for example, have complex life cycles which involve one or more intermediate hosts and must be ingested by the final host to ensure transmission and survival of the parasite organism. Thus, occurrence of a heteroxenic helminth may reflect prey-predator interactions and show the position of hosts in the trophic chain (Brooks & Hoberg, 2001; Marcogliese, 2004). Since parasites can provide a lot of information about host organisms and their habitats, they contribute to the knowledge of host biology, environmental stress, trophic chains and biodiversity (Poulin, 1999; Marcogliese, 2004; Horwitz & Wilcox, 2005). Anjos (2011) highlighted the importance of basic research and ACKNOWLEDGEMENTS Alho, CJR. 1964a. Contribução ao estudo do gênero KlossinemellaCosta, 1961- com descrição de uma nova espécie (Nematoda).Boletim do Museu Paraense Emílio Goeldi, Serie Zoologia, vol. 46, pp. 1-6.Alho, CJR. 1964b. Dois novos hospedadores de Nematophila grande(Diesing, 1839) (Trematoda, Paramphistomoidea. Boletim do Museu Paraense Emílio Goeldi, Serie Zoologia, vol. 52, pp. 1-4.Alho, CJR. 1965. Contribuição ao conhecimento da fauna helmintológica de quelônios do estado do Para, Brasil.Boletim do Museu Paraense Emílio Goeldi, vol. 58, pp.1-8.Alho, CJR & Vicente, JJ. 1964. Podocnemitrema papillosusg. n., sp. n., and a new arrangement of the classification of the family Microscaphidiidae Travassos, 1922 (Trematoda, Paramphistomoidea).Revista Brasileira de Biologia, vol. 24, 1, pp. 17-22.Anderson, R, Chabaud, A & Willmott, S. 2009. Keys to the Nematode Parasites of Vertebrates. CAB International, Wallingford (UK), 480p.Anjos, LA. 2011. Herpetoparasitology in Brazil:BIBLIOGRAPHIC REFERENCES Neotropical Helminthology, 2021, 15(1), ene-junHelminths associated with continental Testudines
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