image/svg+xmlISSN Versión impresa 2218-6425ISSN Versión Electrónica 1995-1043RESEARCH NOTE / NOTA CIENTIFICANEW FRESHWATER FISH HOSTS OF GORDIIDS (NEMATOMORPHA) LARVAE IN THE SOUTH OF CHILENUEVOS PECES DULCEACUICOLAS HOSPEDEROS DE LARVAS DE GORDIACEOS (NEMATOMORPHA) EN EL SUR DE CHILEInstituto de Parasitología, Facultad de Medicina, Edificio de Ciencias Biomédicas, IslaTeja, Universidad Austral de Chile, Valdivia, Chile*Corresponding author: ptorres@uach.clPatricioTorres-Hevia: https://orcid.org./0000-0002-9882-01831*PatricioTorres-HeviaNeotropical Helminthology79doi:10.24039/rnh20201511026Neotropical Helminthology, 2021, 15(1), ene-jun:79-84.DABSTRACTKeywords:Aplochiton– Chile – fish – Nematomorpha – parasites – TrichomycterusNematomorpha Vejdovsky, 1886 includes most of its species in the taxon Gordiida, presenting a freshwater free-living adult and a parasitic larval stage that live in some aquatic invertebrates, amphibians and fish. Present results documented morphological characteristics of unencysted and encysted larvae of gordiids in the intestines of two new freshwater fish hosts, Aplochiton zebra Jenyns, 1842 (Osmeriformes, Galaxiidae) and Trichomycterus areolatusValenciennes, 1846 (Siluriformes: Trichomycteridae), in the south of Chile.RESUMENPalabras clave: Aplochiton– Chile – Nematomorpha – parásitos – peces – TrichomycterusNematomorpha Vejdovsky, 1886 incluye la mayoría de sus especies en el taxón Gordiida, incluyendo un estado adulto de vida libre dulceacuícola y un estado larvario parasítico que vive en algunos invertebrados acuáticos, anfibios y peces. Los resultados del presente estudio documentan las características morfológicas de larvas enquistadas y no enquistadas de gordiáceos en dos nuevos peces hospederos dulceacuícolas, Aplochiton zebraJenyns, 1842 (Osmeriformes, Galaxiidae) y Trichomycterus areolatusValenciennes, 1846 (Siluriformes, Trichomycteridae), en el sur de Chile.
image/svg+xml80The Phylum Nematomorpha Vejdovsky,1886 includes around 350 species distributed in the Gordiida, with freshwater species, and Nectonematida taxa with five marine species (Hanelt et al., 2005; Szmygiel et al., 2014). Gordiida species, commonly called horsehair worms, in their adult stage reproduce in freshwater environments where they lay eggs that develop into larvae (Hanelt et al., 2005). The larvae hatch and establish as parasites after being ingested by paratenic hosts, including aquatic insects (Coleoptera, Diptera, Trichoptera and Ephemeroptera), snails, annelids, crustaceans (amphipods and copepods), amphibians and fish. In these hosts, larvae secrete a cystic wall when located in their tissues or body cavity (Poinar & Doelman, 1974; Hanelt & Janovy, 2003; Hanelt et al., 2012). Aquatic insects predated by terrestrial arthropods (orthopterans, coleopterans and mantids) are infected by the larval stage. As they develop into their adul stage, host behavior is manipulated by the parasite so that they look for and jumps into water; here, the parasite abandon their host to mate (Bolek & Coggins, 2002; Hanelt & Janovy, 2003; Hanelt et al., 2012; Barquin et al., 2015).The records of encysted or unencysted gordiids in fish are scarce and are related to natural infections by G. aquaticusin Misgurnus fossilis(Linnaeus, 1758), Lampetrafluviatilis (Linnaeus, 1758), Lampetra planeri(Bloch, 1784), Thymallus thymallus(Linnaeus, 1758), Phoxinus phoxinus(Linnaeus, 1758), and Salmosp. (Villot, 1881; Von Linstow, 1898; Malmqvist & Moravec, 1978), as well as infections by Gordiussp. in Barbatula barbatula(Linnaeus, 1758) (syn. Barbatulus barbatulus), Rutilus rutilus(Linnaeus, 1758), Leuciscus idus(Linnaeus, 1758), and Carassius carassius(Linnaeus, 1758) (Zhokhov & Molodozhnikova, 2008), in Europe. Additionally, the experimental infection by Gordius robustusLeidy, 1851, P. variusand Chordodes morganiMontgomery, 1898 has been verified in Notropis stramineus(Cope, 1865) (syn. Notropis ludibundus) in the USA (Hanelt & Janovy, 2003). Natural infections by unidentified gordiid cysts was identified in GalaxiasvulgarisStokell, 1949 and Gobiomorphus breviceps(Stokell, 1939) in INTRODUCTION New Zealand (Blair, 1983), and in Galaxias maculatus(Jenyns, 1842) in Chile (Torres et al., 2017).The objective of this research note is to report and describe the morphology of gordiid larvae in two new fish hosts in the Lingue River and Riñihue Lake, in the south of Chile.During January 2014 and 2015, 12 fish were collected distributed in four Aplochiton zebraJenyns, 1842 (Osmeriformes, Galaxiidae) [three in the Lingue River (39°26´60´´S; 73°13´60´´W): one female and two with undetermined sex (US), with 9.3-10.6 cm of body length (BL) and 1 female with 10.8 cm BL in the Riñihue Lake (39°50´36´´S; 72°17´39´´W)], and eight Trichomycterus areolatusValenciennes,1846 (Siluriformes: Trichomycteridae) [three females in the Lingue River with 6.0-6.9 cm BL and two females and 3 males in the Riñihue Lake with 4.5-6.7 cm BL]. All the fish were caught, with authorization from the Subsecretaria de Pesca y Acuicultura (Resolution No. 2430/2013), by electric fishing and taken alive to the Institute of Parasitology, kept in aquariums at 4°C until dissection and examination within 48 hr after its capture. Gordiids were searched in the stomach, intestines, liver, spleen and gonads. The stomach and intestines were opened longitudinally and cut into 2-3 cm long sections. With the contents of the stomach and intestines of each section, preparations were made between slides and coverslips and observing in a light microscope with 40X and 100X. Subsequently, each section was placed between slides and coverslips adding saline solution with slight compression, examining with a light microscope. The other organs were minced and then made preparations between slides and coverslips with slight compression, observing under the microscope. Number of encysted and unencysted larvae of gordiids was determined. The morphological characteristics registered correspond to those proposed by Hanelt & Janovy (2002). Voucher specimens were deposited in the Collection of the Museo de Zoología, Universidad Neotropical Helminthology, 2021, 15(1), ene-junMATERIAL AND METHODSTorres-Hevia
image/svg+xml81de Concepción, Chile.Ethic aspects: The research adhered to ethical aimed at reducing the minimum possible damage to the animals under study as well as the application of biosafety standards in field and laboratory work.The number of infected / examined fish (range of parasite number / infected fish) was as follows for each host and locality: A. zebra: Lingue River 1/1 (280 unencysted larvae), Riñihue Lake 2/3 (7-11 encysted larvae ); T. areolatus: Lingue River 2/3 (7-12 encysted larvae), Riñihue Lake 5/5 (1-122 encysted and unencysted larvae). All gordiids were alive in the intestines of the hosts. Table 1 and Fig.1 show the morphological characteristics of encysted and unencysted larvae. In other studies, in G.vulgaris and G.cotidianus, encysted larvae have been recorded in the intestines, esophagus, stomach, and liver (Blair, RESULTS ANS DISCUSSION1983), in T. thymallusand an unidentified species of Salmo(Villot, 1881) and N. ludibundus(Hanelt & Janovy, 2003) in intestines; and in G. maculatusin intestines and liver (Torres et al., 2017). For Gordius robustus, P. variusand C. morganiin experimental infections of N. stramineus, the encysted larvae were found in the intestines, observing a similar response from the host, including the formation of granulomas around the cysts, which in some cases could destroy them (Hanelt & Janovy, 2003). In G. maculatus, the presence of inflammatory infiltrate associated with gordiid encysted larvae was not observed, however, although most of them were viable, a smaller proportion showed dead larvae (Torres et al., 2017).The characteristics of unencysted larvae (measurements of the preseptum and postseptum, the absence of longer outer hooks on the outer ring of the preseptum, and the presence of only one terminal spine in the postseptum) and encysted ones (larvae folded twice without spines on the preseptum and encysted larvae length) in both hosts (Table 1) suggest they correspond to the Gordiusgenus according to the descriptions of the Neotropical Helminthology, 2021, 15(1), ene-junTable 1. Morphological characteristics (mean and range in µm) of larvae and cysts of gordiids in Aplochiton zebra12Jenyns, 1842 and Trichomycterus areolatusValenciennes, 1846 from Lingue River and Riñihue Lake in the south of Chile.CharacteristicsA. zebra1A. zebra2T. areolatus1T. areolatus2Unencysted larvae n= 5n = 8n= 7Preseptum length39.5 (30-45)44.7 (16-59)35.2(19-44)Preseptum width17.8 (14-20)17.0 (11-22)16.9 (11-21)Postseptum length 100.4 (62-121)97.2 (19-161)83.1(45-110)Postseptum width16.2 (14-18)14.9 (9-20)16.3 (9-21)Stylet length21.9 (17-31)23.6 (11-35)17.4 (10-21)Stylet width6.9 (5-9)10.6 (9-14)4.9 (4-7)Pseudointestine length28.5 (18-39)31.2 (18-46)30.9 (20-38)Pseudointestine width19.8 (12-27) 10.6 (9-14)9.8 (8-12)Encyst larvaen= 6n= 5Total length52.5 (40-70)66.5 (49-98)Total width41.7 (30-55)26.1 (19-41)Cyst wall length13.1 (9-18)14.3 (8-22)Cyst wall width13.8 (9-19)3.2 (2-6)Cyst larva length26.5 (21-35)37.9 (28-54)Cyst larva width14.1 (10-18)19.8 (15-28)Cyst larval folding22Gordiids (Nematomorpha) larvae in fish
image/svg+xml82unencysted and encysted Gordiusspp. larvae in the northern hemisphere (Hanel & Janovy, 2002; Szmygiel et al., 2014; Harkins et al., 2016). Previously, in the Valdivia River basin, where the Riñihue Lake is located, a female of Gordiusgenus was registered, which could correspond to G. robustus(Schmidt-Rhaesa, 2016).The new hosts of gordiids consume different prey through which they could acquire the infection, for example, A. zebrais a consumer of chironomids, Ephemeroptera or Trichoptera while T. areolatusconsumes gastropods, oligochaetes, small crustaceans and insects, such as Ephemeroptera (Ruiz & Marchant, 2004), all groups registered as paratenic hosts (Poinar & Doelman,1974; Hanelt & Janovy, 2003; Hanelt et al., 2012). In Chile, nematomorphs have been scarcely investigated, describing only seven species in their adult stage, among them Beatogordius latastei(Camerano, 1895), Neochordodes talensisFigure 1. A. Unencysted larva of gordiid found in the intestine of Aplochiton zebrafrom Lingue River (arrow: terminal spine). B. Unencysted larva of gordiid found in the intestine of A. zebrafrom Riñihue Lake. C. Encysted larva found in the intestine of Trichomycterus areolatusfrom Lingue River. D. Encysted larva found in the intestine of T. areolatusfrom Riñihue Lake (arrow: terminal spine). Abbreviations: Pre = preseptum. Po = postseptum. Ps = pseudointestine. S = stylet. Neotropical Helminthology, 2021, 15(1), ene-junTorres-Hevia
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